ArticleViruses2023
Development and Implementation of a Quadruple RT-qPCR Method for the Identification of Porcine Reproductive and Respiratory Syndrome Virus Strains.
Article in Viruses, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 17 citations in OpenAlex.
- Development and Application of a Triplex RT-qPCR Assay for Differentiating Major Lineages of Porcine Reproductive and Respiratory Syndrome Virus.Animals : an open access journal from MDPI · 2026Article
- An advanced rapid-visual CRISPR assay for detecting porcine reproductive and respiratory syndrome virus.Scientific reports · 2026Article
- Systematic review and meta-analysis on the diagnostic accuracy of various detection methods for porcine reproductive and respiratory syndrome virus.Porcine health management · 2026Article
- The updated one-step multiplex RT-qPCR method for PRRSV classical strains, highly pathogenic strains and NADC30-like strains.Frontiers in microbiology · 2026Article
- Development of a novel duplex crystal digital PCR for the detection of PRRSV-1 and PRRSV-2.Frontiers in cellular and infection microbiology · 2026Article
- Modified epitope peptides targeting the porcine PD-1/PD-L1 interaction enhance cellular and humoral immune responses.Journal of veterinary science · 2025Article
- Article
- Advancements in Modern Nucleic Acid-Based Multiplex Testing Methodologies for the Diagnosis of Swine Infectious Diseases.Veterinary sciences · 2025Review
- Detection and genetic evolution analysis of porcine reproductive and respiratory syndrome virus in Chengdu from January 2021 to December 2022.Journal of veterinary science · 2025Article
- Article
- A Simple and Sensitive RT-qPCR Technology for Rapid Detection of Porcine Reproductive and Respiratory Syndrome Virus.Veterinary sciences · 2025Article
- Genetic and Pathogenic Characteristics of an Emerging Lineage 7 PRRSV-2 (Prime Pac-Like) Strain in China.Transboundary and emerging diseases · 2025Article
- Development of a Multiplex RT-qPCR Method for the Identification and Lineage Typing of Porcine Reproductive and Respiratory Syndrome Virus.International journal of molecular sciences · 2024Article
- Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPorcine reproductive and respiratory syndrome virus (PRRSV) causes porcine reproductive and respiratory syndrome (PRRS), leading to abortion in sows and respiratory distress in breeding pigs. In China, PRRSV1 and PRRSV2 are the two circulating genotypes in swine herds, with distinct virulence. PRRSV2 further consists of classical (C-PRRSV2), highly pathogenic (HP-PRRSV2), and NADC30-Like (N-PRRSV2) subtypes. The diversity of PRRSV poses challenges for control and eradication, necessitating reliable detection assays for differentiating PRRSV genotypes.
methodsA new TaqMan-based RT-qPCR assay with four sets of primers and probes targeting conserved regions of the ORF7 and NSP2 genes of PRRSV was developed, optimized, and evaluated by us. Reaction conditions such as annealing temperature, primer concentration, and probe concentration were optimized for the assay. Specificity, sensitivity, repeatability, stability, limit of detection (LOD), concordance with the reference method were evaluated for the assay.
resultsThe assay could detect and type PRRSV1, C-PRRSV2, HP-PRRSV2, and N-PRRSV2 simultaneously with 97.33% specificity, 96.00% sensitivity, 12 copies/μL LOD, 97.00% concordance with reference assays. We applied the assay to 321 clinical samples from swine farms in China. The assay successfully detected and typed 230 PRRSV-positive samples, with 24.78% (57/230) of them further confirmed by ORF5 gene sequencing. The prevalence of PRRSV subtypes among the positive samples was as follows: C-PRRSV2 (15.22%), HP-PRRSV2 (23.48%), and N-PRRSV2 (61.30%). Two samples showed coinfection with different PRRSV subtypes.
conclusionThe quadruple RT-qPCR assay is a powerful tool for detecting and typing the currently circulating PRRSV strains in Chinese swine populations. It can assist in the surveillance of PRRSV prevalence and the implementation of prevention and control strategies.
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