Evidence map›Paper›PMID 37765466›Full record

ArticlePlants (Basel, Switzerland)2023

Cryopreservation of Duckweed Genetic Diversity as Model for Long-Term Preservation of Aquatic Flowering Plants.

Anton Peterson, Olena Kishchenko, Markus Kuhlmann, Henning Tschiersch, Joerg Fuchs, Natalia Tikhenko, Ingo Schubert, Manuela Nagel

Abstract read
In one paragraph

Article in Plants (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Cryopreservation by Vitrification for the Aquatic Plant Family Lemnaceae.Methods in molecular biology (Clifton, N.J.) · 2026
    Article
  5. Genetic Stability and Photosystem II Functioning of In Vitro-RecoveredInternational journal of molecular sciences · 2025
    Article
  6. Article
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Anton PetersonLeibniz Institute of Plant Genetics and Crop Plant Research (IPK) OT Gatersleben (ROR (Research Organization Registry)-ID of IPK: https://ror.org/02skbsp27), Corrensstraße 3, 06466 Seeland, Germany.ORCID 0000-0002-1633-5388
Olena KishchenkoLeibniz Institute of Plant Genetics and Crop Plant Research (IPK) OT Gatersleben (ROR (Research Organization Registry)-ID of IPK: https://ror.org/02skbsp27), Corrensstraße 3, 06466 Seeland, Germany.ORCID 0000-0003-4768-2822
Markus KuhlmannLeibniz Institute of Plant Genetics and Crop Plant Research (IPK) OT Gatersleben (ROR (Research Organization Registry)-ID of IPK: https://ror.org/02skbsp27), Corrensstraße 3, 06466 Seeland, Germany.ORCID 0000-0003-3104-0825
Henning TschierschLeibniz Institute of Plant Genetics and Crop Plant Research (IPK) OT Gatersleben (ROR (Research Organization Registry)-ID of IPK: https://ror.org/02skbsp27), Corrensstraße 3, 06466 Seeland, Germany.ORCID 0000-0001-8343-1946
Joerg FuchsLeibniz Institute of Plant Genetics and Crop Plant Research (IPK) OT Gatersleben (ROR (Research Organization Registry)-ID of IPK: https://ror.org/02skbsp27), Corrensstraße 3, 06466 Seeland, Germany.ORCID 0000-0003-4171-5371
Natalia TikhenkoLeibniz Institute of Plant Genetics and Crop Plant Research (IPK) OT Gatersleben (ROR (Research Organization Registry)-ID of IPK: https://ror.org/02skbsp27), Corrensstraße 3, 06466 Seeland, Germany.ORCID 0000-0002-8860-775X
Ingo SchubertLeibniz Institute of Plant Genetics and Crop Plant Research (IPK) OT Gatersleben (ROR (Research Organization Registry)-ID of IPK: https://ror.org/02skbsp27), Corrensstraße 3, 06466 Seeland, Germany.ORCID 0000-0002-6300-2068
Manuela NagelLeibniz Institute of Plant Genetics and Crop Plant Research (IPK) OT Gatersleben (ROR (Research Organization Registry)-ID of IPK: https://ror.org/02skbsp27), Corrensstraße 3, 06466 Seeland, Germany.ORCID 0000-0003-0396-0333

Funding

Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) 491250510Federal Ministry of Education and Research 100632287Philip Schwartz Initiative of the Alexander von Humboldt Foundation 0901324Volkswagen Foundation Az.: 9C069
6 · The paper itself

Abstract

Vegetatively propagating aquatic angiosperms, the Lemnaceae family (duckweeds) represents valuable genetic resources for circular bioeconomics and other sustainable applications. Due to extremely fast growth and laborious cultivation of in vitro collections, duckweeds are an urgent subject for cryopreservation. We developed a robust and fast DMSO-free protocol for duckweed cryopreservation by vitrification. A single-use device was designed for sampling of duckweed fronds from donor culture, further spin-drying, and subsequent transferring to cryo-tubes with plant vitrification solution 3 (PVS3). Following cultivation in darkness and applying elevated temperatures during early regrowth stage, a specific pulsed illumination instead of a diurnal regime enabled successful regrowth after the cryopreservation of 21 accessions of

Indexed as

CBF/DREB1 genescryopreservationduckweedPVS3the operating efficiency of photosystem IIvitrification

Identifiers

PMID37765466
PMCPMC10534739

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.