ArticleMolecules (Basel, Switzerland)2023
Hydrophilic Partitioning or Surface Adsorption? A Quantitative Assessment of Retention Mechanisms for Hydrophilic Interaction Chromatography (HILIC).
Article in Molecules (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Exploring Green-Synthesized Silver Nanoparticles in Neurodegeneration: a Systematic Review of Cholinesterase Enzyme Interactions.Molecular neurobiology · 2026Pooled it
- Systematic Evaluation of Partition-Dominated Retention in Hydrophilic Interaction Liquid Chromatography (HILIC) Under Varying Aqueous Components of the Mobile Phase: Revisiting the HILIC Column Test Scheme.Journal of separation science · 2026Article
- A Novel HILIC-UPLC-DAD-MS/MS Method for the Analysis of Mycosporine-Like Amino Acids and Their Quantification in Diverse Algae.Electrophoresis · 2026Article
- [Preparation of a novel polyhydroxy silica stationary phase and application in the separation of alginate oligosaccharides].Se pu = Chinese journal of chromatography · 2026Article
- Simultaneous ManNAc and Neu5Ac Quantification in Human Sera by LC-MS/MS.International journal of molecular sciences · 2026Article
- Rapid Glass Wool Enrichment of Glycopeptides for MALDI-MS Analysis of Immunoglobulin G Glycosylation in COVID-19 Samples.Journal of the American Society for Mass Spectrometry · 2025Article
- Applications of Hydrophilic Interaction Chromatography in Pharmaceutical Impurity Profiling: A Comprehensive Review of Two Decades.Molecules (Basel, Switzerland) · 2025Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Retention mechanisms in HILIC have been investigated and reported in literature. However, the current understanding of retention mechanisms is qualitative and lacks quantitative details. Previously, mechanism elucidation was based on indirect evidence, and unambiguous assignment of retention mechanisms has not been reported based on direct data. This study aims to quantitatively determine the contributions of two major retention mechanisms in HILIC, hydrophilic partitioning and surface adsorption to the overall retention of neutral compounds. Using the methodologies we developed previously, the phase ratio for adsorbed water layer and distribution coefficients were measured and used to calculate the retention factors contributed by hydrophilic partitioning. The methodology allows the determination of the contribution of surface adsorption simultaneously. The evaluation of five test compounds demonstrates that the retention may be controlled by hydrophilic partitioning, surface adsorption or both depending on compound characteristics. Quantitative assessment of retention mechanisms also makes it possible to better understand the effect of acetonitrile on retention in HILIC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.