ArticleMicroorganisms2023
A High-Throughput Microtiter Plate Screening Assay to Quantify and Differentiate Species in Dual-Species Biofilms.
Article in Microorganisms, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 20 citations in OpenAlex.
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- Measuring Communication in Microbial Biofilms in Response to Antibiotics, Phytochemicals and Stressors.Antioxidants (Basel, Switzerland) · 2026Article
- Interplay ofVirulence · 2025Article
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- Genomic and Phenotypic Characterization of Pseudomonas juntendi Strain A3e, a Drug-Resistant Isolate from Irrigation Water.Current microbiology · 2025Article
- Fundamental changes in the antimicrobial resistance profile of Klebsiella quasipneumoniae ATCC 700603 in response to meropenem stress.BMC microbiology · 2025Article
- MvfR ShapesCells · 2025Article
- An In Vitro Artificial Wound Slough-Biofilm Model Developed for Evaluating a Novel Antibiofilm Technology.Microorganisms · 2024Article
- New tools to monitorFrontiers in cellular and infection microbiology · 2024Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Pathogenic bacteria form biofilms during infection, and polymicrobial biofilms are the most frequent manifestation. Biofilm attachment, maturation, and/or antibiotic sensitivity are mainly evaluated with microtiter plate assays, in which bacteria are stained to enable the quantification of the biomass by optical absorbance or fluorescence emission. However, using these methods to distinguish different species in dual-species or polymicrobial biofilms is currently impossible. Colony-forming unit counts from homogenized dual-species biofilms on selective agar medium allow species differentiation but are time-consuming for a high-throughput screening. Thus, reliable, feasible, and fast methods are urgently needed to study the behavior of polymicrobial and dual-species communities. This study shows that
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Registered trials
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