Evidence map›Paper›PMID 37756257›Full record

ArticleJournal of the American Society for Mass Spectrometry2023

Development of a PNGase Rc Column for Online Deglycosylation of Complex Glycoproteins during HDX-MS.

Thomas Lambert, Marius Gramlich, Luisa Stutzke, Luke Smith, Dingyu Deng, Philipp D Kaiser, Ulrich Rothbauer, Justin L P Benesch, Cornelia Wagner, Maximiliane Koenig and 4 more

Open access · hybridAbstract read
In one paragraph

Article in Journal of the American Society for Mass Spectrometry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 6 institutions in 4 countries.

Thomas LambertDepartment of Pharmacy, University of Copenhagen, 2100 Copenhagen, Denmark.
Marius GramlichNMI Natural and Medical Sciences Institute at the University of Tübingen, 72770 Reutlingen, Germany.
Luisa StutzkeDepartment of Pharmacy, University of Copenhagen, 2100 Copenhagen, Denmark.
Luke SmithPhysical and Theoretical Chemistry Laboratory, Department of Chemistry, University of Oxford, OX1 3QZ Oxford, England.
Dingyu DengDepartment of Pharmacy, University of Copenhagen, 2100 Copenhagen, Denmark.
Philipp D KaiserNMI Natural and Medical Sciences Institute at the University of Tübingen, 72770 Reutlingen, Germany.
Ulrich RothbauerNMI Natural and Medical Sciences Institute at the University of Tübingen, 72770 Reutlingen, Germany.
Justin L P BeneschPhysical and Theoretical Chemistry Laboratory, Department of Chemistry, University of Oxford, OX1 3QZ Oxford, England.ORCID 0000-0002-1507-3742
Cornelia WagnerRoche Pharma Research and Early Development, Large Molecule Research, Roche Innovation Center Munich, 82377 Penzberg, Germany.
Maximiliane KoenigRoche Pharma Research and Early Development, Large Molecule Research, Roche Innovation Center Munich, 82377 Penzberg, Germany.
Petr PompachBioCev, Institute of Biotechnology of the CAS, 252 50 Prumyslova, Czech Republic.
Petr NovakBioCeV, Institute of Microbiology of the CAS, 142 20 Prumyslova, Czech Republic.ORCID 0000-0001-8688-529X
Anne ZeckNMI Natural and Medical Sciences Institute at the University of Tübingen, 72770 Reutlingen, Germany.
Kasper D RandDepartment of Pharmacy, University of Copenhagen, 2100 Copenhagen, Denmark.ORCID 0000-0002-6337-5489
Natural and Medical Sciences Institute · DEUniversity of Copenhagen · DKRoche Pharma AG (Germany) · DEUniversity of Oxford · GBCzech Academy of Sciences, Institute of Biotechnology · CZCzech Academy of Sciences, Institute of Microbiology · CZ

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protein glycosylation is one of the most common PTMs and many cell surface receptors, extracellular proteins, and biopharmaceuticals are glycosylated. However, HDX-MS analysis of such important glycoproteins has so far been limited by difficulties in determining the HDX of the protein segments that contain glycans. We have developed a column containing immobilized PNGase Rc (from

Indexed as

GlycoproteinsPolysaccharidesGlycosylationPeptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine AmidaseGlycoproteinsPeptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine AmidasePolysaccharides

Identifiers

PMID37756257
PMCPMC10623573
OpenAlexW4387093007

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.