Evidence map›Paper›PMID 37751692›Full record

ArticleCell reports methods2023

Three-dimensional multi-color optical nanoscopy at sub-10-nm resolution based on small-molecule organic probes.

Yubing Han, Shijie Tu, Wenwen Gong, Wenli Tao, Mingwei Tang, Yunfei Wei, Cuifang Kuang, Xu Liu, Yu-Hui Zhang, Xiang Hao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Silactins and Structural Diversity of Biosilica in Sponges.Biomimetics (Basel, Switzerland) · 2024
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yubing HanCollege of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, China; Britton Chance Center for Biomedical Photonics-MoE Key Laboratory for Biomedical Photonics, Advanced Biomedical Imaging Facility-Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China. Electronic address: hanyubing@zju.edu.cn.
Shijie TuCollege of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, China.
Wenwen GongCollege of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, China; Institute of Pharmacology, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, P.R. China.
Wenli TaoCollege of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, China.
Mingwei TangCollege of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, China.
Yunfei WeiBritton Chance Center for Biomedical Photonics-MoE Key Laboratory for Biomedical Photonics, Advanced Biomedical Imaging Facility-Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
Cuifang KuangCollege of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, China; Ningbo Research Institute, Zhejiang University, Ningbo, Zhejiang 315100, China.
Xu LiuCollege of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, China; Ningbo Research Institute, Zhejiang University, Ningbo, Zhejiang 315100, China. Electronic address: liuxu@zju.edu.cn.
Yu-Hui ZhangBritton Chance Center for Biomedical Photonics-MoE Key Laboratory for Biomedical Photonics, Advanced Biomedical Imaging Facility-Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China. Electronic address: zhangyh@mail.hust.edu.cn.
Xiang HaoCollege of Optical Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, China. Electronic address: haox@zju.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Achieving nanometer-scale resolution remains challenging in expansion microscopy due to photon loss. To address this concern, here we develop a multi-color expansion stimulated emission depletion technique based on small-molecule probes to realize high labeling density and intensity. Our method substantially lowers the barrier to visualizing diverse intracellular proteins and their interactions in three dimensions. It enables us to achieve sub-10-nm resolution in structures such as microfilaments, lysosomes, and mitochondria, providing new insights into cell biology.

Indexed as

MicroscopyMitochondriaActin CytoskeletonCP: Imaging, super-resolution imaging, expansion microscopy, small-molecule organic probes, subcellular structures, interactions

Identifiers

PMID37751692
PMCPMC10545905

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.