ArticleCell reports methods2023
Assessing crosstalk in simultaneous multicolor single-molecule localization microscopy.
Article in Cell reports methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Discerning protein pools by selective staining with self-labeling tags.FEBS letters · 2026Review
- NanorulerQA: quantitative quality analysis of dual-color DNA nanorulers via single-molecule photobleaching step counting and spatio-temporal colocalization.Biomedical optics express · 2026Article
- Electrochemical Dye Switching Assisted Spectral Demixing and 3D STORM Imaging.Angewandte Chemie (International ed. in English) · 2026Article
- CC-DenseSTORM: deep learning enables colorimetry camera-based simultaneous two-color single-molecule localization microscopy with dense emitters.Biomedical optics express · 2026Article
- Deciphering the Nanoscale Architecture of Presynaptic Actin Using a Micropatterned Presynapse-on-Glass Model.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Spectrin condensates provide a nidus for assembling the axonal membrane-associated periodic skeleton.iScience · 2026Article
- Versatile Microfluidics Platform for Enhanced Multitarget Super-Resolution Microscopy.ACS nano · 2026Article
- Single-molecule localization microscopy imaging of extracellular vesicle DNA in recipient cells.Journal of translational medicine · 2026Article
- Single molecule orientation and localization microscopy.Nature photonics · 2025Article
- Trans-synaptic molecular context of NMDA receptor nanodomains.Nature communications · 2025Article
- Unveiling the cell biology of hippocampal neurons with dendritic axon origin.The Journal of cell biology · 2025Article
- Super-resolution imaging of the neuronal cytoskeleton.Npj imaging · 2024Review
- NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals.Nature communications · 2024Article
- Actin-driven nanotopography promotes stable integrin adhesion formation in developing tissue.Nature communications · 2024Article
- Distinct SAP102 and PSD-95 Nano-organization Defines Multiple Types of Synaptic Scaffold Protein Domains at Single Synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Distinct SAP102 and PSD-95 nano-organization defines multiple types of synaptic scaffold protein domains at single synapses.bioRxiv : the preprint server for biology · 2023Article
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Authors and funding
7 authors.
Funding
Abstract
Single-molecule localization microscopy (SMLM) can reach sub-50 nm resolution using techniques such as stochastic optical reconstruction microscopy (STORM) or DNA-point accumulation for imaging in nanoscale topography (PAINT). Here we implement two approaches for faster multicolor SMLM by splitting the emitted fluorescence toward two cameras: simultaneous two-color DNA-PAINT (S2C-DNA-PAINT) that images spectrally separated red and far-red imager strands on each camera, and spectral demixing dSTORM (SD-dSTORM) where spectrally close far-red fluorophores appear on both cameras before being identified by demixing. Using S2C-DNA-PAINT as a reference for low crosstalk, we evaluate SD-dSTORM crosstalk using three types of samples: DNA origami nanorulers of different sizes, single-target labeled cells, or cells labeled for multiple targets. We then assess if crosstalk can affect the detection of biologically relevant subdiffraction patterns. Extending these approaches to three-dimensional acquisition and SD-dSTORM to three-color imaging, we show that spectral demixing is an attractive option for robust and versatile multicolor SMLM investigations.
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