ArticleMagnetic resonance in medicine2024
Evaluation of the molecular origin of amide proton transfer-weighted imaging.
Article in Magnetic resonance in medicine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Interpreting amide proton transfer-weighted imaging contrast between normal and tumor brain tissues using the asymmetry analysis method at 4.7 T.Magnetic resonance in medicine · 2026Article
- Amide mapping in the human brain using downfield MRSI at 3 T and 7 T.Magnetic resonance in medicine · 2025Article
- Improving quantification accuracy of a nuclear Overhauser enhancement signal at -1.6 ppm at 4.7 T using a machine learning approach.Physics in medicine and biology · 2025Article
- Enhancing amide proton transfer imaging in ischemic stroke using a machine learning approach with partially synthetic data.NMR in biomedicine · 2025Article
- Unraveling contributions to the Z-spectrum signal at 3.5 ppm of human brain tumors.Magnetic resonance in medicine · 2024Article
- A Denoising Convolutional Autoencoder for SNR Enhancement in Chemical Exchange Saturation Transfer imaging: (DCAE-CEST).bioRxiv : the preprint server for biology · 2024Article
- Hippocampal amide proton transfer values are associated with cerebral small vessel disease imaging markers and total burden: a community-based cross-sectional study.Quantitative imaging in medicine and surgery · 2024Article
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3 authors.
Funding
Abstract
purposeTo evaluate the assumption in amide proton transfer weighted (APTw) imaging that the APT dominates over the relayed nuclear Overhauser enhancement (rNOE) and other CEST effects such as those from amines/guanidines, thereby providing imaging of mobile proteins/peptides.
methodsWe introduced two auxiliary asymmetric analysis metrics that can vary the relative contributions from amine/guanidinium CEST and other effects. By comparing these metrics with the conventional asymmetric analysis metric on healthy rat brains, we can approximately assess the contribution from amines/guanidines to APTw and determine whether the APT dominates over the rNOE effect. To further investigate the molecular origin of APTw, we used samples of dialyzed tissue homogenates to eliminate small metabolites and supernatants of homogenates to separate lipids from other components.
resultsWhen the APTw signal is positive using high saturation amplitudes (e.g., 2-3 μT), the contributions from amines/guanidines are significant and cannot be ignored. The APTw signal from the dialyzed homogenates and the controls has negligible changes, indicating that it primarily originates from macromolecules rather than small metabolites. Additionally, the APTw signals with low saturation amplitudes (e.g., 1 μT) were negative in tissue homogenates but positive in their supernatants, suggesting that proteins contribute positively to APTw signals, whereas lipids contribute negatively to it.
conclusionThe positive APTw signal using high saturation amplitudes could have significant contributions from soluble proteins through CEST, including amide/amine/guanidine proton transfer effects. In contrast, the negative APTw signal using low saturation amplitudes has significant contribution from lipids through rNOE.
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