Evidence map›Paper›PMID 37745551›Full record

ArticlebioRxiv : the preprint server for biology2023

Multiplexed CRISPR gene editing in primary human islet cells with Cas9 ribonucleoprotein.

Romina J Bevacqua, Weichen Zhao, Emilio Merheb, Seung Hyun Kim, Alexander Marson, Anna L Gloyn, Seung K Kim

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Romina J BevacquaDepartment of Developmental Biology, Stanford University School of Medicine, Stanford, CA, 94305, USA.
Weichen ZhaoDepartment of Developmental Biology, Stanford University School of Medicine, Stanford, CA, 94305, USA.
Emilio MerhebDiabetes, Obesity and Metabolism Institute (DOMI), Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Seung Hyun KimDepartment of Developmental Biology, Stanford University School of Medicine, Stanford, CA, 94305, USA.
Alexander MarsonGladstone-UCSF Institute of Genomic Immunology and Northern California JDRF Center of Excellence, University of California at San Francisco, CA, 94158, USA.
Anna L GloynDepartment of Pediatrics (Endocrinology) and of Genetics, Stanford University School of Medicine, Stanford, CA, 94305, USA.
Seung K KimDepartment of Developmental Biology, Stanford University School of Medicine, Stanford, CA, 94305, USA.
Northern California Research · USChild Health and Development Institute · USGladstone Institutes · USStanford University · US

Funding

Vanderbilt Diabetes Research CenterP30DK020593 · NIDDK · VANDERBILT UNIVERSITY MEDICAL CENTER · PI OWEN P MCGUINNESS · 2012 to 2026
$29.3M
The Human Islet Distribution Coordinating Center (UC4)UC4DK098085 · NIDDK · BECKMAN RESEARCH INSTITUTE/CITY OF HOPE · PI EVANS-MOLINA, CARMELLA, NILAND, JOYCE CAROL · 2012 to 2017
$25.6M
Stanford Islet Research CoreP30DK116074 · NIDDK · STANFORD UNIVERSITY · PI Seung K Kim · 2017 to 2026
$19.5M
Integrated Islet Distribution Program (U24) - 2021U24DK098085 · NIDDK · BECKMAN RESEARCH INSTITUTE/CITY OF HOPE · PI Carmella Evans-Molina, Joyce Carol Niland · 2021 to 2026
$17.8M
Bridging the gap between type 2 diabetes GWAS and therapeutic targetsUM1DK126185 · NIDDK · UNIV OF NORTH CAROLINA CHAPEL HILL · PI CLAUSSNITZER, MELINA C, GLOYN, ANNA LOUISE · 2020 to 2024
$9.5M
Therapeutic targeting of human islets with recombinant regulatory T cellsU01DK123743 · NIDDK · STANFORD UNIVERSITY · PI KIM, SEUNG K, MEYER, EVERETT · 2019 to 2022
$2.9M
Regulation of gastrointestinal hormone signaling and metabolism by Neuromedin UR01DK107507 · NIDDK · STANFORD UNIVERSITY · PI KIM, SEUNG K · 2017 to 2021
$2.0M
Discovering genetic and hormonal mechanisms underlying diabetes risk from flies to humansR01DK108817 · NIDDK · STANFORD UNIVERSITY · PI KIM, SEUNG K · 2018 to 2022
$2.0M
NIDDK NIH HHS P30 DK020593NIDDK NIH HHS P30 DK116074NIDDK NIH HHS R01 DK107507NIDDK NIH HHS R01 DK108817NIDDK NIH HHS U01 DK123743NIDDK NIH HHS U24 DK098085NIDDK NIH HHS UC4 DK098085NIDDK NIH HHS UM1 DK126185Wellcome Trust
6 · The paper itself

Abstract

Successful genome editing in primary human islets could reveal features of the genetic regulatory landscape underlying β cell function and diabetes risk. Here, we describe a CRISPR-based strategy to interrogate functions of predicted regulatory DNA elements using electroporation of a complex of Cas9 ribonucleoprotein (Cas9 RNP) and guide RNAs into primary human islet cells. We successfully targeted coding regions including the

Identifiers

PMID37745551
PMCPMC10516051
OpenAlexW4386812964

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.