Evidence map›Paper›PMID 37741199›Full record

ArticleVirology2023

Human neurotropic polyomavirus, JC virus, late coding region encodes a novel nuclear protein, ORF4, which targets the promyelocytic leukemia nuclear bodies (PML-NBs) and modulates their reorganization.

A Sami Saribas, Anna Bellizzi, Hassen S Wollebo, Thomas Beer, Hsin-Yao Tang, Mahmut Safak

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 2 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

A Sami SaribasDepartment of Microbiology, Immunology, and Inflammation, Laboratory of Molecular Neurovirology, MERB-757, Lewis Katz School of Medicine at Temple University, 3500 N. Broad Street, Philadelphia, PA 19140, USA.
Anna BellizziDepartment of Microbiology, Immunology, and Inflammation, Laboratory of Molecular Neurovirology, MERB-757, Lewis Katz School of Medicine at Temple University, 3500 N. Broad Street, Philadelphia, PA 19140, USA.
Hassen S WolleboDepartment of Microbiology, Immunology, and Inflammation, Laboratory of Molecular Neurovirology, MERB-757, Lewis Katz School of Medicine at Temple University, 3500 N. Broad Street, Philadelphia, PA 19140, USA.
Thomas BeerThe Wistar Institute Proteomics and Metabolomics Facility Room 252, 3601 Spruce Street, Philadelphia, PA 19104, USA.
Hsin-Yao TangThe Wistar Institute Proteomics and Metabolomics Facility Room 252, 3601 Spruce Street, Philadelphia, PA 19104, USA.
Mahmut SafakDepartment of Microbiology, Immunology, and Inflammation, Laboratory of Molecular Neurovirology, MERB-757, Lewis Katz School of Medicine at Temple University, 3500 N. Broad Street, Philadelphia, PA 19140, USA. Electronic address: msafak@temple.edu.
Temple University · USThe Wistar Institute · US

Funding

Tumor Microenvironment and MetastasisP30CA010815 · NCI · WISTAR INSTITUTE · PI Aaron Robert Goldman · 1985 to 2026
$75.9M
Advancing Cancer Research through Comprehensive Proteomics and Metabolomics AnalysesR50CA221838 · NCI · WISTAR INSTITUTE · PI Hsin-Yao Tang · 2017 to 2026
$2.1M
Regulatory Roles of Agnoprotein in Biology of JC virusR01NS090949 · NINDS · TEMPLE UNIV OF THE COMMONWEALTH · PI SAFAK, MAHMUT · 2015 to 2019
$1.9M
NCI NIH HHS P30 CA010815NCI NIH HHS R50 CA221838NINDS NIH HHS R01 NS090949
6 · The paper itself

Abstract

We previously reported the discovery and characterization of two novel proteins (ORF1 and ORF2) generated by the alternative splicing of the JC virus (JCV) late coding region. Here, we report the discovery and partial characterization of three additional novel ORFs from the same coding region, ORF3, ORF4 and ORF5, which potentially encode 70, 173 and 265 amino acid long proteins respectively. While ORF3 protein exhibits a uniform distribution pattern throughout the cells, we were unable to detect ORF5 expression. Surprisingly, ORF4 protein was determined to be the only JCV protein specifically targeting the promyelocytic leukemia nuclear bodies (PML-NBs) and inducing their reorganization in nucleus. Although ORF4 protein has a modest effect on JCV replication, it is implicated to play major roles during the JCV life cycle, perhaps by regulating the antiviral response of PML-NBs against JCV infections and thus facilitating the progression of the JCV-induced disease in infected individuals.

Indexed as

JC VirusLeukoencephalopathy, Progressive MultifocalPolyomavirusHumansNuclear ProteinsOpen Reading FramesPromyelocytic Leukemia Nuclear BodiesNuclear ProteinsBKVDNA replicationInterferonJCV, SV40Merkel cell carcinoma virusORF, splicingPapillomavirusPML-NBsPolyomavirusProgressive multifocal leukoencephalopathyPromyelocytic leukemia nuclear bodiesRNA splicingTranscription

Identifiers

PMID37741199
PMCPMC10602023
OpenAlexW4386003473

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.