ArticleScience advances2023
An antibody-based molecular switch for continuous small-molecule biosensing.
Article in Science advances, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 48 citations in OpenAlex.
- Continuous Molecular Monitoring Using Electrochemical Aptamer-Based Sensors: Remaining Challenges for Long-Term In Vivo Deployment.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Beyond Detection Limits: Integrated Biosensors for Molecular Diagnostics, Longitudinal Monitoring, and Clinical Translation.Biosensors · 2026Review
- Wearable Electronics for Precision Diagnosis Through Advanced Manufacturing and Integration.Nano-micro letters · 2026Review
- Flexible ACEK-Enhanced Capacitive Aptasensor for Rapid Cortisol Detection in Sweat.Micromachines · 2026Article
- Homogeneous Antibody-DNA Conjugates Using Unmodified Oligonucleotides and Photo-Cross-Linkable Protein G-HUH Endonuclease Fusion Proteins.Bioconjugate chemistry · 2026Article
- Continuous monitoring of blood-interstitial fluid intercompartmental molecular kinetics in freely moving animals.Science advances · 2026Article
- A 3D-Printed Scaffolded Hydrogel Microneedle Array Biosensor for Real-Time, Continuous Monitoring.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Single-molecule readout of reversible nanoswitches enables continuous monitoring of low biomarker concentrations.Nature communications · 2026Article
- Photothermal Recycling Biosensing for Continuous, Sensitive Molecular Quantification.bioRxiv : the preprint server for biology · 2026Article
- Optimizing Surface Functionalization for Aptameric Graphene Nanosensors in Undiluted Physiological Media.Sensors (Basel, Switzerland) · 2026Article
- A refined phenomenological model of viscoelastic clot formation and lysis in trauma-induced coagulopathy.Frontiers in cardiovascular medicine · 2026Article
- Challenges and opportunities of wearable molecular sensors in endocrinology and metabolism.Nature reviews. Endocrinology · 2026Review
- Article
- Implantable bioelectronics and wearable sensors for kidney health and disease.Nature reviews. Nephrology · 2025Review
- Understanding Fast and Slow Signal Changes in a Competitive Particle-Based Continuous Biosensor.Analytical chemistry · 2025Article
- Continuous Protein Sensing Using Fast-Dissociating Antibody Fragments in Competition-Based Biosensing by Particle Motion.ACS sensors · 2025Article
- Generalizable Molecular Switch Designs forAccounts of chemical research · 2025Article
- A Review on Perception of Binding Kinetics in Affinity Biosensors: Challenges and Opportunities.ACS omega · 2025Review
- How Highly Heterogeneous Sensors with Single-Molecule Resolution can Result in Robust Continuous Monitoring Over Long Time Spans.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Continuous Biosensing to Monitor Acute Systemic Inflammation, a Diagnostic Need for Therapeutic Guidance.ACS sensors · 2025Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 2 institutions in 1 country.
Funding
Abstract
We present a generalizable approach for designing biosensors that can continuously detect small-molecule biomarkers in real time and without sample preparation. This is achieved by converting existing antibodies into target-responsive "antibody-switches" that enable continuous optical biosensing. To engineer these switches, antibodies are linked to a molecular competitor through a DNA scaffold, such that competitive target binding induces scaffold switching and fluorescent signaling of changing target concentrations. As a demonstration, we designed antibody-switches that achieve rapid, sample preparation-free sensing of digoxigenin and cortisol in undiluted plasma. We showed that, by substituting the molecular competitor, we can further modulate the sensitivity of our cortisol switch to achieve detection at concentrations spanning 3.3 nanomolar to 3.3 millimolar. Last, we integrated this switch with a fiber optic sensor to achieve continuous sensing of cortisol in a buffer and blood with <5-min time resolution. We believe that this modular sensor design can enable continuous biosensor development for many biomarkers.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.