ArticleThe ISME journal2023
Humic acid-dependent respiratory growth of Methanosarcina acetivorans involves pyrroloquinoline quinone.
Article in The ISME journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed, 18 citations in OpenAlex.
- The RNF/NQR redox pumps: a versatile system for energy transduction in bacteria and archaea.Applied microbiology and biotechnology · 2025Review
- Cometabolism of ferrihydrite reduction and methyl-dismutating methanogenesis byApplied and environmental microbiology · 2025Article
- Humin oxidation drives microbial dehalogenation in oligotrophic environments.The ISME journal · 2025Article
- Anoxygenic photoautotrophy driven by humus and microplastics in a photosynthetic bacterium.ISME communications · 2025Article
- Enhancing PQQ production inFrontiers in microbiology · 2025Article
- A hybrid photocatalytic system enables direct glucose utilization for methanogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Comment on "Humic acid-dependent respiratory growth of Methanosarcina acetivorans involves pyrroloquinoline quinone" by Yuanxu Song et al.The ISME journal · 2024Article
- Response to comment on "Humic acid-dependent respiratory growth of Methanosarcina acetivorans involves pyrroloquinoline quinone" by Yuanxu Song et al.The ISME journal · 2024Article
- High qualityISME communications · 2024Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although microbial humus respiration plays a critical role in organic matter decomposition and biogeochemical cycling of elements in diverse anoxic environments, the role of methane-producing species (methanogens) is not well defined. Here we report that a major fraction of humus, humic acid reduction enhanced the growth of Methanosarcina acetivorans above that attributed to methanogenesis when utilizing the energy sources methanol or acetate, results which showed both respiratory and fermentative modes of energy conservation. Growth characteristics with methanol were the same for an identically cultured mutant deleted for the gene encoding a multi-heme cytochrome c (MmcA), results indicating MmcA is not essential for respiratory electron transport to humic acid. Transcriptomic analyses revealed that growth with humic acid promoted the upregulation of genes annotated as cell surface pyrroloquinoline quinone (PQQ)-binding proteins. Furthermore, PQQ isolated from the membrane fraction was more abundant in humic acid-respiring cells, and the addition of PQQ improved efficiency of the extracellular electron transport. Given that the PQQ-binding proteins are widely distributed in methanogens, the findings extend current understanding of microbial humus respiration in the context of global methane dynamics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.