ArticleMethods and protocols2023
A Robust and Standardized Approach to Quantify Wound Closure Using the Scratch Assay.
Article in Methods and protocols, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Wound Healing Assay.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Integrated in vitro and in silico evaluation of rutin as a potential modulator of wound healing pathways.Molecular diversity · 2026Article
- Comparative Bioassay-Guided Fractionation ofNutrients · 2026Article
- Chlorophyll-Loaded Castor Oil Nanoemulsions Exhibit Photodynamic Therapy Efficacy Against B16-F10 Melanoma with Low Cytotoxicity Toward HaCaT Keratinocytes.Pharmaceuticals (Basel, Switzerland) · 2026Article
- Development and Characterization of a Silver Nanoparticle-Based Hydrogel Containing Hyaluronic Acid and Allantoin for Antibacterial Burn Treatment.Pharmaceutics · 2026Article
- The In Vitro Wound-Scratch Assay: Applications, Technical Advances, and Limitations in Wound Healing Research.International wound journal · 2026Review
- Crosslinking-Dependent Design of Hyaluronic Acid Matrices for Enhanced Bioadhesion and Cellular Response.Pharmaceutics · 2026Article
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- Scratching in style: 3D printers as plotters for automated and complex wound-healing assays.iScience · 2025Article
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- A novel method for evaluating and visualizing scratch wound healing assays using level-set and image sector analysis.PNAS nexus · 2025Article
- Potentials and Challenges in Development of Vesicular Phospholipid Gel as a Novel Dermal Vehicle for Thymol.Pharmaceutics · 2025Article
- Improvement in Transient Agarose Spot (TAS) Cell Migration Assay: Microplate-Based Detection and Evaluation.International journal of molecular sciences · 2025Article
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- Self-Assembly of a Conjugate of Lipoic Acid With a Collagen-Stimulating Pentapeptide Showing Cytocompatibility and Wound Healing Properties, and Chemical and Photolytic Disassembly.Journal of peptide science : an official publication of the European Peptide Society · 2025Article
- Self-Assembled Nanoparticles of Silicon (IV)-NO Donor Phthalocyanine Conjugate for Tumor Photodynamic Therapy in Red Light.Pharmaceutics · 2024Article
- The Role of Fibroblasts in Skin Homeostasis and Repair.Biomedicines · 2024Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The scratch assay is an in vitro assay that allows for high-throughput quantification of wound closure by keratinocytes and fibroblasts with relative ease. However, this assay is amenable to experimental variables, which can result in false-positive and false-negative data, making the interpretation of such data difficult. Also, data variability decreases the sensitivity of the scratch assay. Here, we identify important sources of data variation in the scratch assay and provide rational mitigation strategies that enable robust and highly reproducible quantification of scratch width and area, and ultimately the scratch closure rates. By eliminating these sources of variability, the sensitivity of the scratch assay is enhanced, thereby allowing for identification of dependent variables with wide-ranging impacts on wound closure in a robust and standardized manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.