Evidence map›Paper›PMID 37736956›Full record

ArticleMethods and protocols2023

Novel Protocol for the Preparation of Porcine Bone Marrow Primary Cell Culture for African Swine Fever Virus Isolation.

Olga Puzankova, Vera Gavrilova, Roman Chernyshev, Ivan Kolbin, Alexey Igolkin, Alexandr Sprygin, Ilya Chvala, Ali Mazloum

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods and protocols, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.0field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Olga PuzankovaFGBI Federal Centre for Animal Health, 600901 Vladimir, Russia.
Vera GavrilovaFGBI Federal Centre for Animal Health, 600901 Vladimir, Russia.
Roman ChernyshevFGBI Federal Centre for Animal Health, 600901 Vladimir, Russia.ORCID 0000-0003-3604-7161
Ivan KolbinFGBI Federal Centre for Animal Health, 600901 Vladimir, Russia.
Alexey IgolkinFGBI Federal Centre for Animal Health, 600901 Vladimir, Russia.
Alexandr SpryginFGBI Federal Centre for Animal Health, 600901 Vladimir, Russia.ORCID 0000-0001-5982-3675
Ilya ChvalaFGBI Federal Centre for Animal Health, 600901 Vladimir, Russia.ORCID 0000-0002-1659-3256
Ali MazloumFGBI Federal Centre for Animal Health, 600901 Vladimir, Russia.ORCID 0000-0002-5982-8393
All-Russian Research Institute for Animal Health · RU

Funding

Ministry of Education and Science of Russia 075-15-2021-1054
6 · The paper itself

Abstract

Isolation of African swine fever virus (ASFV) is a critical step towards the identification, titration, characterization, and even modification of the virus. Therefore, it is important to identify a suitable cell line that supports the efficient replication of ASFV for these purposes. This should be achieved even when starting with a low virus load, as in the case of isolating the virus from field samples. This article presents a detailed protocol on the preparation of porcine bone marrow primary (PBMP) cell culture, which has a high sensitivity towards ASFV, resulting in high viral yields with a minimal risk of bacterial contamination.

Indexed as

African swine feverporcine bone marrowprimary cell culturevirus isolation

Identifiers

PMID37736956
PMCPMC10514816
OpenAlexW4386121745

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.