ArticleAquacultural engineering2021
Development of an open hardware 3-D printed conveyor device for continuous cryopreservation of non-batched samples.
Article in Aquacultural engineering, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- An open-hardware platform for conservation of amphibian genetic resources based on gelatin capsules.iScience · 2026Article
- A 3D-printed microdevice for dielectrophoretic torque-driven rotation of dielectric microspheres to support development of manipulation systems for zebrafish eggs.Journal of micromechanics and microengineering : structures, devices, and systems · 2025Article
- Evaluation of industrial and consumer 3-D resin printer fabrication of microdevices for quality management of genetic resources in aquatic species.Micro and nano engineering · 2024Article
- Exploring pathways toward open-hardware ecosystems to safeguard genetic resources for biomedical research communities using aquatic model species.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2024Review
- Simulation analysis of high-throughput oyster cryopreservation at three scales of production.Aquaculture international : journal of the European Aquaculture Society · 2023Article
- An automated modular open-technology device to measure and adjust concentration of aquatic sperm samples for cryopreservation.SLAS technology · 2023Article
- The emerging role of open technologies for community-based improvement of cryopreservation and quality management for repository development in aquatic species.Animal reproduction science · 2022Review
- Article
- SIMULATION MODELING OF A HIGH-THROUGHPUT OYSTER CRYOPRESERVATION PATHWAY.Journal of shellfish research · 2022Article
- An Open-Hardware Insemination Device for Small-Bodied Live-Bearing Fishes to Support Development and Use of Germplasm Repositories.Animals : an open access journal from MDPI · 2022Article
- Article
- An open hardware 3-D printed device for measuring tensile properties of thermoplastic filament polymers at cryogenic temperatures.Cryogenics · 2022Article
- A 3D Printed Vitrification Device for Storage in Cryopreservation Vials.Applied sciences (Basel, Switzerland) · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
A great challenge among communities participating in germplasm repository development is to obtain suitable cryopreservation equipment and devices. Commercial programmable freezers are costly and thus unaffordable to many users. Self-made devices have substantial variability among users, resulting in few opportunities for standardization across communities. The development of open hardware with the increasing accessibility of three-dimensional (3-D) printing offers rapid prototyping and easy fabrication of devices by users around the world at low cost. The present study explored the feasibility of developing operational prototypes of 3-D printed motorized cryopreservation devices for continuous freezing of non-batched samples. A controlled cooling conveyor device (CCCD) was designed and fabricated to cryopreserve sperm samples in straws that were loaded onto chain links suspended over liquid nitrogen held in a Styrofoam box. Cooling rates of 5 to 34 °C/min for 0.5-ml French straws were produced by adjusting the height of conveyor chains, slopes, and liquid nitrogen mass. The plunge temperature (-47 °C to -61 °C) was controlled by adjustment of conveyor speed. The cooling curves from the CCCD were comparable to a commercial programmable freezer. There were no significant differences in post-thaw motility of sperm from ornamental (Koi) common carp (
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.