ArticleMolecular plant pathology2023
degQ associated with the degS/degU two-component system regulates biofilm formation, antimicrobial metabolite production, and biocontrol activity in Bacillus velezensis DMW1.
Article in Molecular plant pathology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 25 citations in OpenAlex.
- Comparative genomics approaches to identify genomic regions associated with the antimicrobial activity ofMicrobial genomics · 2026Article
- Transcriptomic Analysis Reveals the Time-Dependent Mechanism of Antifungal Activity inMicroorganisms · 2026Article
- Review
- Tetrameric structure of Bacillus subtilis DegQ and its predicted interaction with the DegS-DegU two-component system.Acta crystallographica. Section F, Structural biology communications · 2025Article
- The PTS EIIB Component Drives Strain-Specific Virulence inMicroorganisms · 2025Article
- Eliciting Clavulanic Acid Biosynthesis: The Impact ofMetabolites · 2025Article
- Antimicrobial Activity ofInternational journal of molecular sciences · 2025Review
- Pyoluteorin-deficient Pseudomonas protegens improves cooperation with Bacillus velezensis, biofilm formation, co-colonizing, and reshapes rhizosphere microbiome.NPJ biofilms and microbiomes · 2024Article
- Strategies for improving fengycin production: a review.Microbial cell factories · 2024Review
- Research advances in the identification of regulatory mechanisms of surfactin production by Bacillus: a review.Microbial cell factories · 2024Review
- degQ associated with the degS/degU two-component system regulates biofilm formation, antimicrobial metabolite production, and biocontrol activity in Bacillus velezensis DMW1.Molecular plant pathology · 2023Article
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gram-positive bacterium Bacillus velezensis strain DMW1 produces a high level of antimicrobial metabolites that can suppress the growth of phytopathogens. We investigated the mechanism used by degQ and the degS/degU two-component system to regulate the biocontrol characteristics of DMW1. When degQ and degU were deleted, the biofilm formation, cell motility, colonization activities, and antifungal abilities of ΔdegQ and ΔdegU were significantly reduced compared to wild-type DMW1. The expression levels of biofilm-related genes (epsA, epsB, epsC, and tasA) and swarming-related genes (swrA and swrB) were all down-regulated. We also evaluated the impact on secondary metabolites of these two genes. The degQ and degU genes reduced surfactin and macrolactin production and up-regulated the production of fengycin, iturin, bacillaene, and difficidin metabolites. The reverse transcription-quantitative PCR results were consistent with these observations. Electrophoretic mobility shift assay and microscale thermophoresis revealed that DegU can bind to the promoter regions of these six antimicrobial metabolite genes and regulate their synthesis. In conclusion, we provided systematic evidence to demonstrate that the degQ and degU genes are important regulators of multicellular behaviour and antimicrobial metabolic processes in B. velezensis DMW1 and suggested novel amenable strains to be used for the industrial production of antimicrobial metabolites.
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