ArticleParasites & vectors2023
First isolation of Rickettsia amblyommatis from Amblyomma mixtum in Colombia.
Article in Parasites & vectors, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 8 citations in OpenAlex.
- Diversity and prevalence of ticks associated to cattle and wild animals in lagoon ecosystems of northern Veracruz, México.Experimental & applied acarology · 2026Article
- Incidental infestations of humans by hard ticks (Acari: Ixodidae) in Colombia: Case reports and record of Amblyomma cajennense sensu stricto.Ticks and tick-borne diseases · 2025Article
- Cross-continental hitchhiking of exotic ticks on human travelers and ensuing public health challenges in the USA.iScience · 2025Article
- Molecular Detection and Characterization of Rickettsia Species in Ixodid Ticks from Selected Regions of Namibia.Microorganisms · 2024Article
- Molecular screening of tick-borne microorganisms in ticks from rural areas of Panama, with the first record of Ehrlichia minasensis in Rhipicephalus microplus from Central America.Veterinary research communications · 2024Article
- Spotted Fever GroupInsects · 2024Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundRickettsiae are obligate intracellular Gram-negative bacteria that are the causative agent of rickettsioses and are spread to vertebrate hosts by arthropods. There are no previous reports of isolation of Rickettsia amblyommatis for Colombia.
methodsA convenience sampling was executed in three departments in Colombia for direct collection of adult ticks on domestic animals or over vegetation. Ticks were screened for the presence of Rickettsia spp. by real-time polymerase chain reaction (qPCR) amplifying the citrate synthase gene (gltA), and the positive sample was processed for isolation and further molecular characterization by conventional PCR. The absolute and relative frequencies were calculated for several tick species variables. All products from conventional PCR were further purified and sequenced by the Sanger technique. Representative sequences of 18 Rickettsia species were downloaded from GenBank. Consensus phylogenetic trees were constructed for the gltA, ompB, ompA, and htrA genes with 1000 replicates, calculating bootstrap values through the maximum likelihood method and the generalized time reversible substitution model in the MEGA 7.0 software program.
resultsOne female Amblyomma mixtum collected on vegetation was amplified by qPCR (gltA), indicating a frequency of 1.6% (1/61) for Rickettsia spp. INFECTION: Sequence analysis of a rickettsial isolate from this tick in BLASTn showed 100% identity with gltA (340 base pairs [bp]), 99.87% for ompB (782 bp), 98.99% for htrA (497 bp), and 100% for ompA (488 bp) to R. amblyommatis. Concatenated phylogenetic analysis confirmed these findings indicating that the isolate is grouped with other sequences of Amblyomma cajennense complex from Panama and Brazil within the R. amblyommatis clade.
conclusionsThis paper describes the isolation and early molecular identification of a R. amblyommatis strain from A. mixtum in Colombia.
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