ArticleInternational journal of radiation oncology, biology, physics2024
Direct Measurements of FLASH-Induced Changes in Intracellular Oxygenation.
Article in International journal of radiation oncology, biology, physics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Global clinical landscape and multidisciplinary translational frontiers of FLASH radiotherapy: a systematic review and visualized bibliometric mapping based on WoSCC and PubMed (2014-2025).Frontiers in medicine · 2026Pooled it
- FLASH radiotherapy: physico-chemical considerations on ionisation tracks, radical reactions, and the role of oxygen.Radiation oncology (London, England) · 2026Review
- For ultra-high dose rate carbon-ion irradiation, comparable beam parameters induce the equivalent cell sparing (FLASH) effect.Journal of radiation research · 2026Article
- Article
- Ultra-low oxygen tension duringbioRxiv : the preprint server for biology · 2026Article
- Article
- Systematic tissue oxygen variation shows the modulation of murine skin radiation toxicity at ultra-high dose rates.Physics in medicine and biology · 2026Article
- Factors Influencing the Biological Effects of FLASH Irradiation.Antioxidants (Basel, Switzerland) · 2025Review
- Intermediate tissue oxygen level is required to observe murine FLASH skin sparing.bioRxiv : the preprint server for biology · 2025Article
- Oxygen Consumption In Vivo by Ultra-High Dose Rate Electron Irradiation Depends Upon Baseline Tissue Oxygenation.International journal of radiation oncology, biology, physics · 2025Article
- Oxygen consumption measurements at ultra-high dose rate over a wide LET range.Medical physics · 2025Article
- FLASH radiotherapy combined with immunotherapy: From biological mechanisms to blockbuster therapeutics.Translational oncology · 2025Review
- Dose Rate Effects from the 1950s through to the Era of FLASH.Radiation research · 2024Review
- Navigating the Critical Translational Questions for Implementing FLASH in the Clinic.Seminars in radiation oncology · 2024Review
- Correlation between local instantaneous dose rate and oxygen pressure reduction during proton pencil beam scanning irradiation.Physics and imaging in radiation oncology · 2024Article
- Photoacoustic lifetime oxygen imaging of radiotherapy-induced tumor reoxygenationJournal of photochemistry and photobiology · 2024Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
purposeThe goal of our study was to characterize the dynamics of intracellular oxygen during application of radiation at conventional (CONV) and FLASH dose rates and obtain evidence for or against the oxygen depletion hypothesis as a mechanism of the FLASH effect. METHODS AND MATERIALS: The measurements were performed by the phosphorescence quenching method using probe Oxyphor PtG4, which was delivered into the cellular cytosol by electroporation.
resultsIntracellular radiochemical oxygen depletion (ROD) g-value for a dose rate of 100 Gy/s in the normoxic range was found to be 0.58 ± 0.03 μM/Gy. Intracellular ROD g-values for FLASH and CONV dose rates in the normoxic range were found to be nearly equal. As in solution-based studies, intracellular ROD was found to exhibit strong dependence on oxygen concentration in the range of 0 to ∼40 μM [O
conclusionsDepletion of oxygen in cells in vitro by a clinical dose of proton radiation delivered as FLASH is unable to produce a transient state of hypoxia and, therefore, unable to induce radioprotection. The difference between ROD g-values for FLASH and CONV dose rates, detected previously in solutions-based experiments, disappears when measurements are conducted inside cells. Understanding this phenomenon should provide additional insight into the role of oxygen in FLASH radiation therapy and help to decipher the mechanism of the FLASH effect.
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