ArticleACS synthetic biology2023
C2CAplus: A One-Pot Isothermal Circle-to-Circle DNA Amplification System.
Article in ACS synthetic biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Development of a Microdroplet-Based Functional Genomic Screening Pipeline by Combination of DNA Nanoflowers and PURExpress Cell-Free Expression.ACS synthetic biology · 2026Article
- Systematic Exploration of Synthesis and Function Landscapes for DNA Hydrogels.ACS synthetic biology · 2026Article
- Polymerization-Assisted Signal Enhancement and Visual Readout Techniques in Bioassays: A Mini Review.ACS polymers Au · 2025Review
- Reconstituting alternative life using the test-bed of cell-free systems.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Simultaneous in vitro expression of minimal 21 transfer RNAs by tRNA array method.Nature communications · 2025Article
- Continuous in situ synthesis of a complete set of tRNAs sustains steady-state translation in a recombinant cell-free system.Nature communications · 2025Article
- Post-assembly Plasmid Amplification for Increased Transformation Yields inChem & bio engineering · 2025Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
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Authors and funding
3 authors.
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Abstract
Rolling circle amplification (RCA) is a widely used DNA amplification method that uses circular template DNA as input and produces multimeric, linear single- or double-stranded DNA. Circle-to-circle amplification (C2CA) has further expanded this method by implementing product recircularization using restriction and ligation, leading to a higher amplification yield and enabling the generation of circular products. However, C2CA is a multistep, nonisothermal method, requiring multiple fluid manipulations and thereby compromises several advantages of RCA. Here, we improved C2CA to implement a one-pot, single step, isothermal reaction at temperatures ranging from 25 to 37 °C. Our C2CAplus method is simple, robust, and produces large quantities of product DNA that can be seen with the naked eye.
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