Evidence map›Paper›PMID 37729059›Full record

ArticleSTAR protocols2023

Simultaneous electrophysiology and optogenetic perturbation of the same neurons in chronically implanted animals using μLED silicon probes.

Nathaniel R Kinsky, Mihály Vöröslakos, Jose Roberto Lopez Ruiz, Laurel Watkins de Jong, Nathan Slager, Sam McKenzie, Euisik Yoon, Kamran Diba

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
  2. Article
  3. Erasable Hippocampal Neural Signatures Predict Memory Discrimination.bioRxiv : the preprint server for biology · 2024
    Article
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Nathaniel R KinskyDepartment of Anesthesiology and Neuroscience Graduate Program, University of Michigan Medical School, Ann Arbor, MI 48109, USA. Electronic address: nkinsky@umich.edu.
Mihály VöröslakosDepartment of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI 48109, USA; Neuroscience Institute, Langone Medical Center, New York University, New York, NY 10016, USA.
Jose Roberto Lopez RuizDepartment of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
Laurel Watkins de JongDepartment of Anesthesiology and Neuroscience Graduate Program, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
Nathan SlagerDepartment of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
Sam McKenzieDepartment of Neuroscience, University of New Mexico, Albuquerque, NM 87131, USA.
Euisik YoonDepartment of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; Center for Nanomedicine, Institute for Basic Science (IBS) and Graduate Program of Nano Biomedical Engineering (Nano BME), Advanced Science Institute, Yonsei University, Seoul 03722, South Korea.
Kamran DibaDepartment of Anesthesiology and Neuroscience Graduate Program, University of Michigan Medical School, Ann Arbor, MI 48109, USA. Electronic address: kdiba@umich.edu.

Funding

Div Supp: Daniela del Rio Pulido CRCNS: Unsupervised Learning of Hippocampal Sequence Dynamic in SleepR01NS115233 · NINDS · RICE UNIVERSITY · PI DIBA, KAMRAN, KEMERE, CALEB · 2019 to 2023
$2.3M
Investigation into the synaptic origins of hippocampal replayR00MH118423 · NIMH · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI MCKENZIE, SAMUEL ARNOLD · 2020 to 2022
$923k
CRCNS: US-German Proposal: Mechanisms of Sequence Generation in the HippocampusR01MH109170 · NIMH · UNIVERSITY OF WISCONSIN MILWAUKEE · PI DIBA, KAMRAN · 2015 to 2017
$673k
The Influence of Sleep on Plasticity and Stability in Hippocampal-Prefrontal Networks.F32NS117732 · NINDS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI KINSKY, NATHANIEL REID · 2020 to 2022
$143k
NIMH NIH HHS R00 MH118423NIMH NIH HHS R01 MH109170NINDS NIH HHS F32 NS117732NINDS NIH HHS R01 NS115233
6 · The paper itself

Abstract

Micro-light-emitting-diode (μLED) silicon probes feature independently controllable miniature light-emitting-diodes (LEDs) embedded at several positions in each shank of a multi-shank probe, enabling temporally and spatially precise optogenetic neural circuit interrogation. Here, we present a protocol for performing causal and reproducible neural circuit manipulations in chronically implanted, freely moving animals. We describe steps for introducing optogenetic constructs, preparing and implanting a μLED probe, performing simultaneous in vivo electrophysiology with focal optogenetic perturbation, and recovering a probe following termination of an experiment. For complete details on the use and execution of this protocol, please refer to Watkins de Jong et al. (2023).

Indexed as

OptogeneticsSiliconAnimalsElectrophysiological PhenomenaElectrophysiologyNeuronsSiliconBiotechnology and BioengineeringNeuroscienceSystems Biology

Identifiers

PMID37729059
PMCPMC10510336

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.