Evidence map›Paper›PMID 37728608›Full record

ArticleActa crystallographica. Section F, Structural biology communications2023

Biochemical and X-ray analyses of the players involved in the faRel2/aTfaRel2 toxin-antitoxin operon.

Lucia Dominguez-Molina, Ariel Talavera, Albinas Cepauskas, Tatsuaki Kurata, Dannele Echemendia-Blanco, Vasili Hauryliuk, Abel Garcia-Pino

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta crystallographica. Section F, Structural biology communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 71% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 3 countries.

Lucia Dominguez-MolinaCellular and Molecular Microbiology, Faculté des Sciences, Université Libre de Bruxelles (ULB), Boulevard du Triomphe, Building BC (1C4 203), 1050 Brussels, Belgium.ORCID 0009-0005-4118-3346
Ariel TalaveraCellular and Molecular Microbiology, Faculté des Sciences, Université Libre de Bruxelles (ULB), Boulevard du Triomphe, Building BC (1C4 203), 1050 Brussels, Belgium.
Albinas CepauskasCellular and Molecular Microbiology, Faculté des Sciences, Université Libre de Bruxelles (ULB), Boulevard du Triomphe, Building BC (1C4 203), 1050 Brussels, Belgium.
Tatsuaki KurataDepartment of Experimental Medical Science, Lund University, Lund, Sweden.
Dannele Echemendia-BlancoCellular and Molecular Microbiology, Faculté des Sciences, Université Libre de Bruxelles (ULB), Boulevard du Triomphe, Building BC (1C4 203), 1050 Brussels, Belgium.ORCID 0009-0000-5590-9180
Vasili HauryliukDepartment of Experimental Medical Science, Lund University, Lund, Sweden.ORCID 0000-0003-2389-5057
Abel Garcia-PinoCellular and Molecular Microbiology, Faculté des Sciences, Université Libre de Bruxelles (ULB), Boulevard du Triomphe, Building BC (1C4 203), 1050 Brussels, Belgium.
Université Libre de Bruxelles · BELund University · SE

Funding

Cancerfonden 20 0872 PjCrafoordska Stiftelsen 20220562Eesti Teadusagentuur PRG335European Research Council, H2020 European Research Council CoG DiStRes, No. 864311Fonds De La Recherche Scientifique - FNRS FNRS CDR J.0068.19Fonds De La Recherche Scientifique - FNRS FNRS-EQP UN.025.19Fonds De La Recherche Scientifique - FNRS FNRS PDR T.0066.18Fonds De La Recherche Scientifique - FNRS FNRS PDR T.0090.22Fonds De La Recherche Scientifique - FNRS J.0065.23FFonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture FRIA FC31211Knut och Alice Wallenbergs Stiftelse 2020-0037Vetenskapsrådet 2021-01146
6 · The paper itself

Abstract

The aTfaRel2/faRel2 operon from Coprobacillus sp. D7 encodes a bicistronic type II toxin-antitoxin (TA) module. The FaRel2 toxin is a toxic small alarmone synthetase (toxSAS) that inhibits translation through the pyrophosphorylation of uncharged tRNAs at the 3'-CCA end. The toxin is neutralized by the antitoxin ATfaRel2 through the formation of an inactive TA complex. Here, the production, biophysical analysis and crystallization of ATfaRel2 and FaRel2 as well as of the ATfaRel2-FaRel2 complex are reported. ATfaRel2 is monomeric in solution. The antitoxin crystallized in space group P2

Indexed as

AntitoxinsBacterial ToxinsBacterial ProteinsCrystallography, X-RayOperonX-RaysAntitoxinsBacterial ProteinsBacterial ToxinsalarmonesATfaRel2–FaRel2 complexCoprobacillusRSH proteinstoxin–antitoxin modulestoxSAStRNA modification

Identifiers

PMID37728608
PMCPMC10565793
OpenAlexW4386878835

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.