Evidence map›Paper›PMID 37726827›Full record

ArticleBiomarker research2023

Circulating cell-free DNA (cfDNA) in patients with medullary thyroid carcinoma is characterized by specific fragmentation and methylation changes with diagnostic value.

Anna Citarella, Zein Mersini Besharat, Sofia Trocchianesi, Tanja Milena Autilio, Antonella Verrienti, Giuseppina Catanzaro, Elena Splendiani, Zaira Spinello, Silvia Cantara, Patrizia Zavattari and 10 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in Biomarker research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.2field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 5 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors at 6 institutions in 1 country.

Anna Citarella *Department of Experimental Medicine, Sapienza University of Rome, Rome, 00161, Italy.
Zein Mersini Besharat *Department of Experimental Medicine, Sapienza University of Rome, Rome, 00161, Italy.
Sofia TrocchianesiDepartment of Experimental Medicine, Sapienza University of Rome, Rome, 00161, Italy.
Tanja Milena AutilioDepartment of Experimental Medicine, Sapienza University of Rome, Rome, 00161, Italy.
Antonella VerrientiDepartment of Translational and Precision Medicine, Sapienza University of Rome, Rome, 00161, Italy.
Giuseppina CatanzaroDepartment of Experimental Medicine, Sapienza University of Rome, Rome, 00161, Italy.
Elena SplendianiDepartment of Movement, Human and Health Sciences, University of Foro Italico, 00135, Rome, Italy.
Zaira SpinelloDepartment of Experimental Medicine, Sapienza University of Rome, Rome, 00161, Italy.
Silvia CantaraDepartment of Medical, Surgical and Neurological Sciences, University of Siena, Siena, 53100, Italy.
Patrizia ZavattariDepartment of Biomedical Sciences, Unit of Biology and Genetics, University of Cagliari, Cagliari, 09042, Italy.
Eleonora LoiDepartment of Biomedical Sciences, Unit of Biology and Genetics, University of Cagliari, Cagliari, 09042, Italy.
Cristina RomeiEndocrine Unit, Department of Clinical and Experimental Medicine, University of Pisa, Pisa, 56126, Italy.
Raffaele CiampiEndocrine Unit, Department of Clinical and Experimental Medicine, University of Pisa, Pisa, 56126, Italy.
Luciano PezzulloThyroid Surgical Unit, IRCCS Fondazione G.Pascale, Naples, 80131, Italy.
Maria Grazia CastagnaDepartment of Medical, Surgical and Neurological Sciences, University of Siena, Siena, 53100, Italy.
Antonio AngeloniDepartment of Experimental Medicine, Sapienza University of Rome, Rome, 00161, Italy.
Rosella EliseiEndocrine Unit, Department of Clinical and Experimental Medicine, University of Pisa, Pisa, 56126, Italy.
Cosimo DuranteDepartment of Translational and Precision Medicine, Sapienza University of Rome, Rome, 00161, Italy.
Agnese Po *Department of Molecular Medicine, Sapienza University of Rome, Rome, 00161, Italy. Agnese.Po@uniroma1.it.
Elisabetta Ferretti *Department of Experimental Medicine, Sapienza University of Rome, Rome, 00161, Italy.
Sapienza University of Rome · ITUniversity of Pisa · ITUniversity of Cagliari · ITUniversity of Siena · ITForo Italico University of Rome · ITIstituto Nazionale Tumori IRCCS "Fondazione G. Pascale" · IT

Funding

AIFA (Agenzia Italiana del Farmaco) TRS-2016-00001141
6 · The paper itself

Abstract

Medullary Thyroid Carcinoma (MTC) is a rare neuroendocrine tumour whose diagnosis includes evaluating calcitonin serum levels, which can present fluctuations unrelated to MTC. Here, we investigated circulating DNA fragmentation and methylation changes as potential biomarkers using ddPCR on cell-free DNA (cfDNA) isolated from the plasma of MTC patients. For cfDNA fragmentation analysis, we investigated the fragment size distribution of a gene family and calculated short fragment fraction (SFF). Methylation analyses evaluated the methylation levels of CG_16698623, a CG dinucleotide in the MGMT gene that we found hypermethylated in MTC tissues by analyzing public databases. The SFF ratio and methylation of CG_16698623 were significantly increased in plasma from MTC patients at diagnosis, and patients with clinical remission or stable disease at follow-up showed no significant SFF difference compared with healthy subjects. Our data support the diagnostic value of cfDNA traits that could enable better management of MTC patients.

Indexed as

Cell-free DNACirculating biomarkerCirculating DNA fragmentationCirculating DNA methylationMedullary thyroid carcinomaRare tumour

Identifiers

PMID37726827
PMCPMC10510276
OpenAlexW4386857246

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.