Evidence map›Paper›PMID 37725835›Full record

ArticleDiabetes2023

Phenotypic Characterization of Congenital Hyperinsulinism Due to Novel Activating Glucokinase Mutations.

Changhong Li, Christine A Juliana, Yue Yuan, Ming Li, Ming Lu, Pan Chen, Kara E Boodhansingh, Nicolai M Doliba, Tricia R Bhatti, N Scott Adzick and 2 more

Open access · bronzeAbstract read
In one paragraph

Article in Diabetes, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.0field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 10 citations in OpenAlex.

  1. Trial
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Genetic Variations in Hyperinsulinemic Hypoglycemia: Active versus Inactive Mutations.Diabetes, metabolic syndrome and obesity : targets and therapy · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 2 countries.

Changhong LiDivision of Endocrinology and Diabetes, The Children's Hospital of Philadelphia, Philadelphia, PA.
Christine A JulianaDivision of Endocrinology and Diabetes, The Children's Hospital of Philadelphia, Philadelphia, PA.
Yue YuanNanjing AscendRare Pharmaceutical Technology Co., Nanjing, China.
Ming LiDepartment of Endocrinology, National Health Commission (NHC) Key Laboratory of Endocrinology, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.ORCID 0000-0002-8427-605X
Ming LuDivision of Endocrinology and Diabetes, The Children's Hospital of Philadelphia, Philadelphia, PA.
Pan ChenDivision of Endocrinology and Diabetes, The Children's Hospital of Philadelphia, Philadelphia, PA.
Kara E BoodhansinghDivision of Endocrinology and Diabetes, The Children's Hospital of Philadelphia, Philadelphia, PA.
Nicolai M DolibaInstitute of Diabetes, Obesity and Metabolism, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA.
Tricia R BhattiDepartment of Pathology, The Children's Hospital of Philadelphia, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA.
N Scott AdzickDepartment of Surgery, The Children's Hospital of Philadelphia, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA.
Charles A StanleyDivision of Endocrinology and Diabetes, The Children's Hospital of Philadelphia, Philadelphia, PA.
Diva D De LeónDivision of Endocrinology and Diabetes, The Children's Hospital of Philadelphia, Philadelphia, PA.ORCID 0000-0003-1225-8087
Children's Hospital of Philadelphia · USChinese Academy of Medical Sciences & Peking Union Medical College · CNUniversity of Pennsylvania · US

Funding

VIRAL VECTOR COREP30DK019525 · NIDDK · UNIVERSITY OF PENNSYLVANIA · PI JOSHUA D RABINOWITZ · 1986 to 2026
$48.3M
Neurological Phenotyping in Hyperinsulinism – Administrative Supplement to Islet dysregulation in Infants with Congenital HyperinsulinismR01DK056268 · NIDDK · CHILDREN'S HOSP OF PHILADELPHIA · PI DE LEON, DIVA D., GANGULY, ARUPA · 1999 to 2024
$9.1M
Insulin Secretion in Hyperinsulinism Human IsletsR01DK098517 · NIDDK · CHILDREN'S HOSP OF PHILADELPHIA · PI DE LEON, DIVA D. · 2013 to 2023
$4.5M
NIDDK NIH HHS P30 DK019525NIDDK NIH HHS P30DK19525NIDDK NIH HHS R01 DK056268NIDDK NIH HHS R01 DK098517
6 · The paper itself

Abstract

The importance of glucokinase (GK) in the regulation of insulin secretion has been highlighted by the phenotypes of individuals with activating and inactivating mutations in the glucokinase gene (GCK). Here we report 10 individuals with congenital hyperinsulinism (HI) caused by eight unique activating mutations of GCK. Six are novel and located near previously identified activating mutations sites. The first recognized episode of hypoglycemia in these patients occurred between birth and 24 years, and the severity of the phenotype was also variable. Mutant enzymes were expressed and purified for enzyme kinetics in vitro. Mutant enzymes had low glucose half-saturation concentration values and an increased enzyme activity index compared with wild-type GK. We performed functional evaluation of islets from the pancreata of three children with GCK-HI who required pancreatectomy. Basal insulin secretion in perifused GCK-HI islets was normal, and the response to glyburide was preserved. However, the threshold for glucose-stimulated insulin secretion in perifused glucokinase hyperinsulinism (GCK-HI) islets was decreased, and glucagon secretion was greatly suppressed. Our evaluation of novel GCK disease-associated mutations revealed that the detrimental effects of these mutations on glucose homeostasis can be attributed not only to a lowering of the glucose threshold of insulin secretion but also to a decreased counterregulatory glucagon secretory response. ARTICLE HIGHLIGHTS: Our evaluation of six novel and two previously published activating GCK mutations revealed that the detrimental effects of these mutations on glucose homeostasis can be attributed not only to a lowering of the glucose threshold of insulin secretion but also to a decreased counterregulatory glucagon secretory response. These studies provide insights into the pathophysiology of GCK-hyperinsulinism and the dual role of glucokinase in β-cells and α-cells to regulate glucose homeostasis.

Indexed as

Congenital HyperinsulinismHyperinsulinismChildGlucagonGlucokinaseGlucoseHumansMutationPhenotypeGlucagonGlucokinaseGlucose

Identifiers

PMID37725835
PMCPMC10658072
OpenAlexW4386855530

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.