Evidence map›Paper›PMID 37725792›Full record

ArticleACS synthetic biology2023

Complex Dependence of

Fernanda Piorino, Mark P Styczynski

Open access · hybridAbstract read
In one paragraph

Article in ACS synthetic biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.9field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Fernanda PiorinoSchool of Chemical & Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Drive NW, Atlanta, Georgia 30332-0100, United States.ORCID 0000-0002-9032-1400
Mark P StyczynskiSchool of Chemical & Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Drive NW, Atlanta, Georgia 30332-0100, United States.ORCID 0000-0002-1479-6658
Georgia Institute of Technology · US

Funding

Synthetic biology-based detection of micronutrients with minimal equipmentR01EB022592 · NIBIB · GEORGIA INSTITUTE OF TECHNOLOGY · PI STYCZYNSKI, MARK PHILIP-WALTER · 2017 to 2020
$1.4M
Synthetic biological systems for protein detectionR01EB034301 · NIBIB · GEORGIA INSTITUTE OF TECHNOLOGY · PI Mark Philip-Walter Styczynski · 2023 to 2026
$1.4M
NIBIB NIH HHS R01 EB022592NIBIB NIH HHS R01 EB034301
6 · The paper itself

Abstract

Cell lysis─by sonication or bead beating, for example─is a key step in preparing extracts for cell-free expression systems. To create high protein-production capacity extracts, standard practice is to lyse cells sufficiently to thoroughly disrupt the membrane and thus extract expression machinery but without degrading that machinery. Here, we investigate the impact of different sonication energy inputs on the protein-production capacity of

Indexed as

Escherichia coliSonicationPlasmidscell-free expression systemslysisprotein expressionsonicationvariability

Identifiers

PMID37725792
PMCPMC10594866
OpenAlexW4386856371

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.