ArticleNature methods2023
GelMap: intrinsic calibration and deformation mapping for expansion microscopy.
Article in Nature methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Tissue expansion mass spectrometry imaging (TEMI) for high-spatial-resolution multiomics molecular mapping.Nature protocols · 2026Review
- Article
- Hydrogel Chemistry in Expansion Microscopy: From Polyacrylamide to Next-Generation Swellable Polymers.Chembiochem : a European journal of chemical biology · 2026Review
- Landscape expansion microscopy reveals interactions between membrane and phase-separated organelles.The Journal of cell biology · 2026Article
- Article
- Review of expansion microscopy combined with advanced imaging modalities.Journal of microscopy · 2026Review
- Tensile expansion microscopy applies mechanical force to super-resolve fixed and image live cellular samples.bioRxiv : the preprint server for biology · 2026Article
- Expansion omics: from expansion microscopy to spatial omics.Molecular systems biology · 2026Review
- Visualising viral interactions and mechanisms at the nanoscale with expansion microscopy.Npj viruses · 2026Review
- Expansion and fluctuations-enhanced microscopy for nanoscale molecular profiling of cells and tissues.Nature protocols · 2026Review
- Landscape Expansion Microscopy Reveals Interactions between Membrane and Phase-Separated Organelles.bioRxiv : the preprint server for biology · 2025Article
- Spatial fluorescence barcode by transiently luminescent DNA beads.Nature communications · 2025Article
- µCodes: A Universal Grid Platform for Microscale Mapping, Microscopy Navigation, and Multimodal Imaging.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Combinatorial protein barcodes enable self-correcting neuron tracing with nanoscale molecular context.bioRxiv : the preprint server for biology · 2025Article
- TEMI: tissue-expansion mass-spectrometry imaging.Nature methods · 2025Article
- Diverse reactivity of maleimides in polymer science and beyond.Polymer international · 2025Article
- Article
- Ultrastructure expansion microscopy (U-ExM) of mouse and human kidneys for analysis of subcellular structures.Cytoskeleton (Hoboken, N.J.) · 2024Article
- Expanding Insights: Harnessing Expansion Microscopy for Super-Resolution Analysis of HIV-1-Cell Interactions.Viruses · 2024Article
- HiExM: high-throughput expansion microscopy enables scalable super-resolution imaging.bioRxiv : the preprint server for biology · 2024Article
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Authors and funding
8 authors.
Funding
Abstract
Expansion microscopy (ExM) is a powerful technique to overcome the diffraction limit of light microscopy by physically expanding biological specimen in three dimensions. Nonetheless, using ExM for quantitative or diagnostic applications requires robust quality control methods to precisely determine expansion factors and to map deformations due to anisotropic expansion. Here we present GelMap, a flexible workflow to introduce a fluorescent grid into pre-expanded hydrogels that scales with expansion and reports deformations. We demonstrate that GelMap can be used to precisely determine the local expansion factor and to correct for deformations without the use of cellular reference structures or pre-expansion ground-truth images. Moreover, we show that GelMap aids sample navigation for correlative uses of expansion microscopy. Finally, we show that GelMap is compatible with expansion of tissue and can be readily implemented as a quality control step into existing ExM workflows.
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Registered trials
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