Evidence map›Paper›PMID 37720050›Full record

ArticleResearch square2023

Temporal dynamics and genomic programming of plasma cell fates.

Godhev Kumar Manakkat Vijay, Ming Zhou, Kairavee Thakkar, Abigail Rothrauff, Amanpreet Singh Chawla, Dianyu Chen, Louis Chi-Wai Lau, Peter Habib, Kashish Chetal, Prabal Chhibbar and 7 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

17 authors.

Godhev Kumar Manakkat VijayCenter for Systems Immunology and Department of Immunology, University of Pittsburgh, Pittsburgh, PA, USA.
Ming ZhouKey Laboratory of Growth Regulation and Translational Research of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou 310024, Zhejiang, China.
Kairavee ThakkarDivision of Bioinformatics, Cincinnati Children's Hospital and Medical Center, Cincinnati, Ohio, USA.
Abigail RothrauffCenter for Systems Immunology and Department of Immunology, University of Pittsburgh, Pittsburgh, PA, USA.
Amanpreet Singh ChawlaDivision of Immunobiology, Cincinnati Children's Hospital and Medical Center, Cincinnati, Ohio, USA; Department of Immunology, University of Pittsburgh, Pittsburgh, PA, USA.
Dianyu ChenKey Laboratory of Growth Regulation and Translational Research of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou 310024, Zhejiang, China.
Louis Chi-Wai LauCenter for Systems Immunology and Department of Immunology, University of Pittsburgh, Pittsburgh, PA, USA.
Peter HabibCenter for Systems Immunology and Department of Immunology, University of Pittsburgh, Pittsburgh, PA, USA.
Kashish ChetalDivision of Bioinformatics, Cincinnati Children's Hospital and Medical Center, Cincinnati, Ohio, USA.
Prabal ChhibbarCenter for Systems Immunology and Department of Immunology, University of Pittsburgh, Pittsburgh, PA, USA.
Jingyu FanCenter for Systems Immunology and Department of Immunology, University of Pittsburgh, Pittsburgh, PA, USA.
Jishnu DasCenter for Systems Immunology and Department of Immunology, University of Pittsburgh, Pittsburgh, PA, USA.
Alok JoglekarCenter for Systems Immunology and Department of Immunology, University of Pittsburgh, Pittsburgh, PA, USA.
Lisa BorghesiCenter for Systems Immunology and Department of Immunology, University of Pittsburgh, Pittsburgh, PA, USA.
Nathan SalomonisDivision of Bioinformatics, Cincinnati Children's Hospital and Medical Center, Cincinnati, Ohio, USA.
Heping XuKey Laboratory of Growth Regulation and Translational Research of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou 310024, Zhejiang, China.
Harinder SinghCenter for Systems Immunology and Department of Immunology, University of Pittsburgh, Pittsburgh, PA, USA.ORCID 0000-0002-6330-8526

Funding

Programming Durable Immune Responses To VaccinationU01AI141990 · NIAID · WASHINGTON UNIVERSITY · PI Ali Hassan Ellebedy · 2019 to 2026
$5.3M
B cell population structure in mouse and manR01AI145064 · NIAID · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI SINGH, HARINDER · 2019 to 2022
$1.5M
NIAID NIH HHS R01 AI145064NIAID NIH HHS U01 AI141990
6 · The paper itself

Abstract

Affinity-matured plasma cells (PCs) of varying lifespans are generated through a germinal center (GC) response. The developmental dynamics and genomic programs of antigen-specific PC precursors remain to be elucidated. Using a model antigen, we demonstrate biphasic generation of PC precursors, with those generating long-lived bone marrow PCs preferentially produced in the late phase of GC response. Clonal tracing using scRNA-seq+BCR-seq in spleen and bone marrow compartments, coupled with adoptive transfer experiments, reveal a novel PC transition state that gives rise to functionally competent PC precursors. The latter undergo clonal expansion, dependent on inducible expression of TIGIT. We propose a model for the proliferation and programming of precursors of long-lived PCs, based on extended antigen encounters followed by reduced antigen availability.

Indexed as

BCRseqclonal trackingdurability of PCsPlasma cell precursorsscRNAseqtemporal PC dynamics

Identifiers

PMID37720050
PMCPMC10503833

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.