Evidence map›Paper›PMID 37719077›Full record

ArticleBio-protocol2023

An In-depth Guide to the Ultrastructural Expansion Microscopy (U-ExM) of

Nikolai Klena, Giovanni Maltinti, Umut Batman, Gaia Pigino, Paul Guichard, Virginie Hamel

Abstract read
In one paragraph

Article in Bio-protocol, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Review
  6. Article
  7. Expansion Microscopy in Euglenozoa.Methods in molecular biology (Clifton, N.J.) · 2026
    Article
  8. Review
  9. Article
  10. Article
  11. Review
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Nikolai KlenaHuman Technopole, Milan, Italy.
Giovanni MaltintiHuman Technopole, Milan, Italy.
Umut BatmanDepartment of Molecular and Cellular Biology, Section of Biology, University of Geneva, Geneva, Switzerland.
Gaia PiginoHuman Technopole, Milan, Italy.
Paul GuichardDepartment of Molecular and Cellular Biology, Section of Biology, University of Geneva, Geneva, Switzerland.
Virginie HamelDepartment of Molecular and Cellular Biology, Section of Biology, University of Geneva, Geneva, Switzerland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Expansion microscopy is an innovative method that enables super-resolution imaging of biological materials using a simple confocal microscope. The principle of this method relies on the physical isotropic expansion of a biological specimen cross-linked to a swellable polymer, stained with antibodies, and imaged. Since its first development, several improved versions of expansion microscopy and adaptations for different types of samples have been produced. Here, we show the application of ultrastructure expansion microscopy (U-ExM) to investigate the 3D organization of the green algae

Indexed as

Basal bodyCell architectureChlamydomonasChloroplastExpansion microscopyFlagellaMembranesMicrotubulesMitochondriaSuper-resolution microscopy

Identifiers

PMID37719077
PMCPMC10502176

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.