ArticleThe Journal of cell biology1986
Regulation of glucose-6-phosphate dehydrogenase activity in sea urchin eggs by reversible association with cell structural elements.
Article in The Journal of cell biology, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 59 citations in OpenAlex.
- Development of placental abnormalities in location and anatomy.Acta obstetricia et gynecologica Scandinavica · 2020Pooled it
- Calcium at fertilization and in early development.Physiological reviews · 2006Review
- Localization of glucose-6-phosphate dehydrogenase activity on ribosomes of granular endoplasmic reticulum, in peroxisomes and peripheral cytoplasm of rat liver parenchymal cells.The Histochemical journal · 2001Article
- The dynamics of local kinetic parameters of glutamate dehydrogenase in rat liver.Histochemistry and cell biology · 1996Article
- Kupffer cells and pit cells are not effective in the defense against experimentally induced colon carcinoma metastasis in rat liver.Clinical & experimental metastasis · 1996Article
- Heterogeneity of kinetic parameters of enzymes in situ in rat liver lobules.Histochemistry and cell biology · 1995Review
- Analysis of enzyme reactions in situ.The Histochemical journal · 1995Review
- The diverse Michaelis constants and maximum velocities of lactate dehydrogenase in situ in various types of cell.The Histochemical journal · 1994Article
- Isolation and the complete amino acid sequence of lumenal endoplasmic reticulum glucose-6-phosphate dehydrogenase.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- In situ glucose-6-phosphate dehydrogenase activity during development of pre-implantation mouse embryos.The Histochemical journal · 1993Article
- Stable, resealable pores formed in sea urchin eggs by electric discharge (electroporation) permit substrate loading for assay of enzymes in vivo.Cell regulation · 1989Article
- Polyvinyl alcohol and other tissue protectants in enzyme histochemistry: a consumer's guide.The Histochemical journal · 1989Review
- Enzyme stimulation upon fertilization is revealed in electrically permeabilized sea urchin eggs.Proceedings of the National Academy of Sciences of the United States of America · 1988Article
- In situ kinetic parameters of glucose-6-phosphate dehydrogenase and phosphogluconate dehydrogenase in different areas of the rat liver acinus.The Histochemical journalArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In unfertilized eggs of the sea urchin, Strongylocentrotus purpuratus, glucose-6-phosphate dehydrogenase (G6PDH) associates with the particulate elements remaining either after homogenization or extraction of eggs with non-ionic detergent in low ionic-strength media. At physiological ionic strength, the extent of G6PDH binding to these particulate elements is proportional to the total protein concentration in the extracts. In fertilized eggs this association is prevented by one or more low molecular weight solutes. The dissociation is reversible, and there are no permanent modifications of either G6PDH or its particulate binding site that affect binding. After fertilization, the time course of dissociation of G6PDH from particulate elements is too fast to be caused by a change in intracellular pH, but it could be triggered, but not maintained, by an increase in the intracellular calcium concentration. Binding of G6PDH to the particulate fraction lowers its catalytic activity at all substrate concentrations. Therefore, release of the enzyme into the cytoplasm may be an important part of the suite of events causing metabolic activation of the egg at fertilization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.