Evidence map›Paper›PMID 37714852›Full record

ArticleNPJ microgravity2023

Human skeletal muscle tissue chip autonomous payload reveals changes in fiber type and metabolic gene expression due to spaceflight.

Maddalena Parafati, Shelby Giza, Tushar S Shenoy, Jorge A Mojica-Santiago, Meghan Hopf, Legrand K Malany, Don Platt, Isabel Moore, Zachary A Jacobs, Paul Kuehl and 7 more

Open access · goldAbstract read
In one paragraph

Article in NPJ microgravity, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
5.6field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 32 citations in OpenAlex.

  1. Review
  2. Article
  3. Organ-on-a-chip toxicology.Innovation (Cambridge (Mass.)) · 2026
    Review
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  5. Article
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  7. Article
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  10. Article
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  13. Review
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  16. Microphysiological systems to advance human pathophysiology and translational medicine.Journal of applied physiology (Bethesda, Md. : 1985) · 2024
    Review
  17. Heart-on-a-Chip at the final frontier.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  18. Article
  19. Review
  20. Omics Studies of Tumor Cells under Microgravity Conditions.International journal of molecular sciences · 2024
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

17 authors at 2 institutions in 1 country.

Maddalena Parafati *Department of Pharmacodynamics, College of Pharmacy, University of Florida, Gainesville, FL, 32610, USA.
Shelby Giza *Department of Pharmacodynamics, College of Pharmacy, University of Florida, Gainesville, FL, 32610, USA.ORCID http://orcid.org/0000-0002-0772-8200
Tushar S ShenoyDepartment of Pharmacodynamics, College of Pharmacy, University of Florida, Gainesville, FL, 32610, USA.
Jorge A Mojica-SantiagoJ. Crayton Pruitt Family Department of Biomedical Engineering, Herbert Wertheim College of Engineering, University of Florida, Gainesville, FL, 32610, USA.
Meghan HopfTranslational Research Institute, AdventHealth, Orlando, FL, 32804, USA.
Legrand K MalanyMicro-gRx, INC, Orlando, FL, 32935, USA.
Don PlattMicro Aerospace Solutions, INC, Melbourne, FL, 32935, USA.
Isabel MooreSpace Tango, LLC, Lexington, KY, 40505, USA.
Zachary A JacobsSpace Tango, LLC, Lexington, KY, 40505, USA.
Paul KuehlSpace Tango, LLC, Lexington, KY, 40505, USA.
Jason RexroatSpace Tango, LLC, Lexington, KY, 40505, USA.
Gentry BarnettSpace Tango, LLC, Lexington, KY, 40505, USA.
Christine E SchmidtJ. Crayton Pruitt Family Department of Biomedical Engineering, Herbert Wertheim College of Engineering, University of Florida, Gainesville, FL, 32610, USA.ORCID http://orcid.org/0000-0002-5865-6016
William T McLambSpace Tango, LLC, Lexington, KY, 40505, USA.
Twyman ClementsSpace Tango, LLC, Lexington, KY, 40505, USA.
Paul M CoenTranslational Research Institute, AdventHealth, Orlando, FL, 32804, USA.
Siobhan MalanyDepartment of Pharmacodynamics, College of Pharmacy, University of Florida, Gainesville, FL, 32610, USA. smalany@cop.ufl.edu.ORCID http://orcid.org/0000-0003-0710-7460
University of Florida · USAdventHealth Orlando · US

Funding

Electrical Stimulation of Human Myocytes in Microgravity: An In Vitro Model to Evaluate Therapeutics to Counteract Muscle WastingUG3TR002598 · NCATS · UNIVERSITY OF FLORIDA · PI MALANY, SIOBHAN · 2018 to 2019
$1.0M
NCATS NIH HHS UG3 TR002598
6 · The paper itself

Abstract

Microphysiological systems provide the opportunity to model accelerated changes at the human tissue level in the extreme space environment. Spaceflight-induced muscle atrophy experienced by astronauts shares similar physiological changes to muscle wasting in older adults, known as sarcopenia. These shared attributes provide a rationale for investigating molecular changes in muscle cells exposed to spaceflight that may mimic the underlying pathophysiology of sarcopenia. We report the results from three-dimensional myobundles derived from muscle biopsies from young and older adults, integrated into an autonomous CubeLab™, and flown to the International Space Station (ISS) aboard SpaceX CRS-21 as part of the NIH/NASA funded Tissue Chips in Space program. Global transcriptomic RNA-Seq analyses comparing the myobundles in space and on the ground revealed downregulation of shared transcripts related to myoblast proliferation and muscle differentiation. The analyses also revealed downregulated differentially expressed gene pathways related to muscle metabolism unique to myobundles derived from the older cohort exposed to the space environment compared to ground controls. Gene classes related to inflammatory pathways were downregulated in flight samples cultured from the younger cohort compared to ground controls. Our muscle tissue chip platform provides an approach to studying the cell autonomous effects of spaceflight on muscle cell biology that may not be appreciated on the whole organ or organism level and sets the stage for continued data collection from muscle tissue chip experimentation in microgravity. We also report on the challenges and opportunities for conducting autonomous tissue-on-chip CubeLab

Identifiers

PMID37714852
PMCPMC10504373
OpenAlexW4386765100

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.