Evidence map›Paper›PMID 37713292›Full record

ArticleThe CRISPR journal2023

Enhancing Precision and Efficiency of Cas9-Mediated Knockin Through Combinatorial Fusions of DNA Repair Proteins.

Ryan R Richardson, Marilyn Steyert, Saovleak N Khim, Garrett W Crutcher, Cheryl Brandenburg, Colin D Robertson, Andrea J Romanowski, Jeffrey Inen, Bekir Altas, Alexandros Poulopoulos

Open access · hybridAbstract read
In one paragraph

Article in The CRISPR journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Large Donor CRISPR for Whole-Coding Sequence Replacement of Cell Adhesion Molecule LRRTM2.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025
    Article
  7. Article
  8. Article
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  10. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Ryan R RichardsonDepartment of Pharmacology and UM-MIND, University of Maryland School of Medicine, Baltimore, Maryland, USA.ORCID 0000-0001-8717-2048
Marilyn SteyertDepartment of Pharmacology and UM-MIND, University of Maryland School of Medicine, Baltimore, Maryland, USA.ORCID 0000-0003-4095-4776
Saovleak N KhimDepartment of Pharmacology and UM-MIND, University of Maryland School of Medicine, Baltimore, Maryland, USA.ORCID 0000-0003-1794-9063
Garrett W CrutcherDepartment of Pharmacology and UM-MIND, University of Maryland School of Medicine, Baltimore, Maryland, USA.
Cheryl BrandenburgDepartment of Pharmacology and UM-MIND, University of Maryland School of Medicine, Baltimore, Maryland, USA.
Colin D RobertsonDepartment of Pharmacology and UM-MIND, University of Maryland School of Medicine, Baltimore, Maryland, USA.
Andrea J RomanowskiDepartment of Pharmacology and UM-MIND, University of Maryland School of Medicine, Baltimore, Maryland, USA.ORCID 0000-0001-8920-7400
Jeffrey InenDepartment of Pharmacology and UM-MIND, University of Maryland School of Medicine, Baltimore, Maryland, USA.ORCID 0000-0002-2491-7759
Alexandros PoulopoulosDepartment of Pharmacology and UM-MIND, University of Maryland School of Medicine, Baltimore, Maryland, USA.ORCID 0000-0002-5318-7388
University of Maryland, Baltimore · USTwitter (United States) · US

Funding

Training Grant in Cancer BiologyT32CA154274 · NCI · UNIVERSITY OF MARYLAND BALTIMORE · PI Toni M Antalis, CURT I CIVIN · 2011 to 2026
$6.8M
Multidisciplinary Schizophrenia Research TrainingT32MH067533 · NIMH · UNIVERSITY OF MARYLAND BALTIMORE · PI CARPENTER, WILLIAM T., ELMER, GREGORY I · 2004 to 2025
$4.3M
UMB Postbaccalaureate Research Education ProgramR25GM113262 · NIGMS · UNIVERSITY OF MARYLAND BALTIMORE · PI ANTALIS, TONI M, CAREY, GREGORY B · 2016 to 2025
$3.2M
Untangling the biology of brain wiring: high-throughput innovations for molecular screening and functional targeting of circuit development in vivoDP2MH122398 · NIMH · UNIVERSITY OF MARYLAND BALTIMORE · PI POULOPOULOS, ALEXANDROS · 2019 to 2019
$2.3M
NCI NIH HHS T32 CA154274NIGMS NIH HHS R25 GM113262NIMH NIH HHS DP2 MH122398NIMH NIH HHS T32 MH067533
6 · The paper itself

Abstract

Cas9 targets genomic loci with high specificity. For knockin with double-strand break repair, however, Cas9 often leads to unintended on-target knockout rather than intended edits. This imprecision is a barrier for direct

Indexed as

CRISPR-Cas SystemsGene EditingAnimalsCRISPR-Associated Protein 9DNA Breaks, Double-StrandedDNA RepairMiceCRISPR-Associated Protein 9

Identifiers

PMID37713292
PMCPMC10611978
OpenAlexW4386768687

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.