Evidence map›Paper›PMID 37712274›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2023

[High expression of Circ-PALLD in heart failure is transcriptionally regulated by the transcription factor GATA4].

Z Huang, Z Zeng, J Li, R Cai, W He, S Hu

Abstract readEnglish Abstract
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Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

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5citing papers in PubMed
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3 · Its place in the literature

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5 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Z HuangSchool of Basic Medical Sciences, Ningxia Medical University, Yinchuan 750004, China.
Z ZengDepartment of Heart Medicine, Changhai Hospital, Naval Medical University, Shanghai 200433, China.
J LiDepartment of Heart Medicine, Changhai Hospital, Naval Medical University, Shanghai 200433, China.
R CaiSchool of Basic Medical Sciences, Ningxia Medical University, Yinchuan 750004, China.
W HeDepartment of Heart Medicine, Changhai Hospital, Naval Medical University, Shanghai 200433, China.
S HuSchool of Basic Medical Sciences, Ningxia Medical University, Yinchuan 750004, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo determine the changes in the expression of circular RNA Circ-PALLD in heart failure and explore the biogenesis of Circ-PALLD.

methodsWe analyzed second-generation sequencing results of human and murine heart failure samples to identify the candidate CircRNAs. Sanger generation sequencing was performed after PCR amplification, and the sequencing results were compared to determine the reverse splicing pattern of the corresponding CircRNAs. We further examined the expressions of CircRNAs and linear RNAs in 8 patients with heart failure admitted in our hospital, and RT-qPCR was performed to detect the expression levels of Circ-PALLD and PALLD in the failing myocardium. Bioinformatic analysis was performed to predict the transcription factors that may regulate PALLD. Small interfering RNAs (siRNAs) against GATA4 were used to determine the regulatory effect of the transcription factor GATA4 on PALLD.

resultsSanger sequencing and sequence alignment verified the reverse splicing of Circ-VWA8, Circ-VMP1, Circ-PRDM5, Circ-PLCL2, Circ-PALLD, Circ-NFATC3, Circ-MLIP, Circ-FAM208A, Circ-ANKIB1, and Circ-AGTPBP1, demonstrated their loop-forming nature and determined the exon arrangement of reverse splicing. Semi-quantitative PCR results showed that the expression levels of CircPALLD, Circ-NFATC3 and Circ-AGTPBP1 were significantly increased while the expression level of linear PALLD was significantly decreased in the myocardial tissues of heart failure patients. Bioinformatic analysis suggested that the transcription of PALLD was regulated possibly by the transcription factor GATA4. RT-qPCR showed that the expression level of Circ-PALLD was significantly increased, while PALLD expression was significantly decreased in the failing myocardium, which was consistent with the results of semi-quantitative PCR. In primary mammary rat cardiomyocytes, GATA4 knockdown resulted in lowered expressions of both Circ-PALLD and PALLD.

conclusionCirc-PALLD is highly expressed in heart failure and can be used as a novel molecular marker for chronic heart failure, and GATA4 may play important role in regulating its transcription. Circ-PALLD points a new direction for investigating the molecular mechanism of heart failure and may also serve as a potential therapeutic target for heart failure.

Indexed as

Heart FailureSerine-Type D-Ala-D-Ala CarboxypeptidaseAdenosine TriphosphatasesAnimalsBasic Helix-Loop-Helix Leucine Zipper Transcription FactorsCytoskeletal ProteinsGATA4 Transcription FactorGTP-Binding ProteinsHumansIntracellular Signaling Peptides and ProteinsMembrane ProteinsMiceMyocardiumRatsRNA, CircularRNA, Small InterferingAdenosine TriphosphatasesAGTPBP1 protein, humanBasic Helix-Loop-Helix Leucine Zipper Transcription FactorsCytoskeletal ProteinsGATA4 protein, humanGata4 protein, mouseGATA4 Transcription FactorGTP-Binding ProteinsIntracellular Signaling Peptides and ProteinsMax protein, mouseMembrane ProteinsPalld protein, ratPLCL2 protein, humanRNA, CircularRNA, Small InterferingSerine-Type D-Ala-D-Ala CarboxypeptidaseVMP1 protein, humanVWA8 protein, mousebiomarkerCirc-PALLDGATA4heart failure

Identifiers

PMID37712274
PMCPMC10505580

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