Evidence map›Paper›PMID 37712273›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2023

[MiR-301a-5p modulates parathyroid hormone secretion in secondary hyperparathyroidism possibly by regulating calcium-sensing receptor].

L Liu, L Qian, P Li, J Li, S Huang, W Yi, S Liu, W Wu

Abstract readEnglish Abstract
PubMed Full text at PMC Publisher
In one paragraph

Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

L LiuDepartment of Breast and Thyroid Surgery, Third Xiangya Hospital, Central South University, Changsha 410013, China.
L QianDepartment of Breast and Thyroid Surgery, Third Xiangya Hospital, Central South University, Changsha 410013, China.
P LiDepartment of Breast and Thyroid Surgery, Third Xiangya Hospital, Central South University, Changsha 410013, China.
J LiDepartment of Breast and Thyroid Surgery, Third Xiangya Hospital, Central South University, Changsha 410013, China.
S HuangDepartment of Breast and Thyroid Surgery, Third Xiangya Hospital, Central South University, Changsha 410013, China.
W YiDepartment of Breast and Thyroid Surgery, Second Xiangya Hospital, Central South University, Changsha 410013, China.
S LiuDepartment of Breast and Thyroid Surgery, Third Xiangya Hospital, Central South University, Changsha 410013, China.
W WuDepartment of Breast and Thyroid Surgery, Third Xiangya Hospital, Central South University, Changsha 410013, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo explore the miRNAs that down- regulate calcium-sensing receptor (CaSR) in secondary hyperparathyroidism (SHPT) and their effects on parathyroid hormone (PTH) secretion.

methodsWhole transcriptome sequencing was performed for 6 normal parathyroid tissue samples and 11 SHPT parathyroid tissue samples. Based on bioinformatic prediction, we screened out 7 candidate miRNAs that regulate CaSR, among which the most likely miRNA for CaSR regulation was identified by double luciferase test. We detected the differential expression of miR-301a-5p and CaSR mRNA in SHPT and normal parathyroid tissue using qRT-PCR, and analyzed the correlation between their expressions and serum PTH levels of the patients. Western blotting was used to detect the expression of CaSR protein in primary SHPT parathyroid cells transfected with miR-301a-5p mimics or inhibitors, and the level of PTH in the supernatant of the cell culture was determined.

resultsAmong the preliminarily selected 7 miRNAs that potentially regulate CaSR (miR-15a-5p, miR-15b-5p, miR- 16- 5p, miR- 221- 3p, miR- 222- 3p, miR- 301a- 5p and miR- 503- 5p), miR- 301a-5p was significantly upregulated in SHPT compared with normal parathyroid tissue (

conclusionMiR-301a-5p affects PTH secretion in SHPT possibly by regulating the expression of CaSR.

Indexed as

Hyperparathyroidism, SecondaryMicroRNAsBlotting, WesternHumansParathyroid HormoneReceptors, Calcium-SensingRNA, MessengerCASR protein, humanMicroRNAsMIRN301A microRNA, humanParathyroid HormonePTH protein, humanReceptors, Calcium-SensingRNA, Messengercalcium-sensing receptorsmiR-301a-5pparathyroid hormonesecondary hyperparathyroidism

Identifiers

PMID37712273
PMCPMC10505570

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.