Two-step method for rapid isolation of genomic DNA and validation of R81T insecticide resistance mutation in

Muhammad Umair Sial, Tahir Farooq, Luaay Kahtan Khalaf, Saqib Rahman, Muhammad Asad, Bilal Ahamad Paray

Abstract read

In one paragraph

Article in Saudi journal of biological sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Comparative Assessment of Different Rearing Diets on the Developmental and Reproductive Behaviour of Fall Armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae).Neotropical entomology · 2025
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Muhammad Umair SialDepartment of Entomology, University of Agriculture, Faisalabad 38000, Pakistan.

Tahir FarooqPlant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, PR China.

Luaay Kahtan KhalafDepartment of Plant Protection, College of Agricultural Engineering Science, University of Baghdad, Baghdad, Iraq.

Saqib RahmanState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, PR China.

Muhammad AsadCollege of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.

Bilal Ahamad ParayDepartment of Zoology, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Isolation and amplification of nucleic acid (DNA) is considered a vital and potent instrument in molecular biological research. However, its functioning outside of a laboratory setting is difficult because of complex procedures that demand expert personnel and expensive equipment in addition to the fulfillment of several additional requirements. DNA isolation from minute insects is sometimes difficult, making diagnostic and genotyping procedures problematic. Thus, the current work offers a high-throughput, cost-effective, straightforward, and faster approach for isolating DNA from the aphid

Indexed as

DNAMyzus persicaePhenol:chloroformPurityR81TSalt outYield

Identifiers

PMID37711971

DOI10.1016/j.sjbs.2023.103791

PMCPMC10497781

What OpenQuestion holds

Textmetadata

LicenceCC BY-NC-ND

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.