ReviewNature methods2024
Community-developed checklists for publishing images and image analyses.
Abstract readReview
In one paragraph
Review in Nature methods, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
0numbers the graph read from it
0cells of the map it votes in
45citing papers in PubMed
–field-weighted citation impact
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
45 citing papers in PubMed.
- Ten simple rules for preparing and delivering effective funding pitches in competitive grant applications.PLoS computational biology · 2026Article
- The graphical abstract.Nature methods · 2026Article
- Open and sustainable AI: challenges, opportunities and the road ahead in the life sciences.Nature methods · 2026Review
- A human induced pluripotent stem cell model for the holistic study of epithelial-to-mesenchymal transitions.Nature methods · 2026Article
- Review
- Points of View, anew.Nature methods · 2026Article
- Practical statistics for bioimage analysis - a guide to experimental design and data interpretation.Journal of cell science · 2026Article
- Artificial intelligence-powered microscopy: Transforming the landscape of parasitology.Journal of microscopy · 2026Review
- Advances in Coumarin Fluorescent Probes for Medical Diagnostics: A Review of Recent Developments.Biosensors · 2026Review
- Reproducibility roadblocks and standardization in carbon dot synthesis: a critical review of current practices, challenges, and future directions.Mikrochimica acta · 2025Review
- Article
- Article
- Best practices in plant fluorescence imaging and reporting: A primer.The Plant cell · 2025Review
- A checklist for designing and improving the visualization of scientific data.Nature cell biology · 2025Article
- Addressing persistent challenges in digital image analysis of cancer tissue: resources developed from a hackathon.Molecular oncology · 2025Review
- Reporting light microscopy data in our pages.Nature structural & molecular biology · 2025Article
- Colony context and size-dependent compensation mechanisms give rise to variations in nuclear growth trajectories.Cell systems · 2025Article
- Morphological profiling data resource enables prediction of chemical compound properties.iScience · 2025Article
- Bridging imaging-based in vitro methods from biomedical research to regulatory toxicology.Archives of toxicology · 2025Review
- Integrating bioengineering, super-resolution microscopy and mechanobiology in autophagy research: addendum to the guidelines (4th edition).Autophagy · 2025Article
Corrections and comments
- Update of
Authors and funding
54 authors.
Christopher SchmiedFondazione Human Technopole, Milano, Italy. schmied@fmp-berlin.de.ORCID http://orcid.org/0000-0003-2058-1124
Michael S NelsonDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.ORCID http://orcid.org/0000-0003-0480-5597
Sergiy AvilovMax Planck Institute of Immunobiology and Epigenetics, Freiburg, Germany.ORCID http://orcid.org/0000-0003-0860-8857
Gert-Jan BakkerMedical BioSciences Department, Radboud University Medical Centre, Nijmegen, the Netherlands.ORCID http://orcid.org/0000-0003-3602-0014
Cristina BertocchiLaboratory for Molecular Mechanics of Cell Adhesions, Pontificia Universidad Católica de Chile Santiago, Santiago de Chile, Chile.ORCID http://orcid.org/0000-0003-0907-1318
Johanna BischofEuro-BioImaging ERIC, Bio-Hub, Heidelberg, Germany.ORCID http://orcid.org/0000-0002-5668-841X
Ulrike BoehmCarl Zeiss AG, Oberkochen, Germany.ORCID http://orcid.org/0000-0001-7471-2244
Jan BrocherScientific Image Processing and Analysis, BioVoxxel, Ludwigshafen, Germany.ORCID http://orcid.org/0000-0001-8565-625X
Mariana T CarvalhoNanophotonics and BioImaging Facility at INL, International Iberian Nanotechnology Laboratory, Braga, Portugal.ORCID http://orcid.org/0000-0002-4281-5799
Catalin ChiritescuPhi Optics, Inc., Champaign, IL, USA.
Jana ChristopherBiochemistry Center Heidelberg, Heidelberg University, Heidelberg, Germany.ORCID http://orcid.org/0000-0002-2699-3368
Beth A CiminiImaging Platform, Broad Institute, Cambridge, MA, USA.ORCID http://orcid.org/0000-0001-9640-9318
Eduardo Conde-Sousai3S, Instituto de Investigação e Inovação Em Saúde and INEB, Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal.ORCID http://orcid.org/0000-0002-6591-5063
Michael EbnerLeibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany.ORCID http://orcid.org/0000-0003-4234-7187
Rupert EckerTranslational Research Institute, Queensland University of Technology, Woolloongabba, Queensland, Australia.ORCID http://orcid.org/0000-0002-1095-8592
Kevin EliceiriDepartment of Medical Physics and Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.ORCID http://orcid.org/0000-0001-8678-670X
Julia Fernandez-RodriguezCentre for Cellular Imaging Core Facility, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.ORCID http://orcid.org/0000-0003-4522-0966
Nathalie GaudreaultAllen Institute for Cell Science, Seattle, WA, USA.ORCID http://orcid.org/0000-0002-9220-5366
Laurent GelmanFriedrich Miescher Institute for Biomedical Research, Basel, Switzerland.ORCID http://orcid.org/0000-0003-3277-3115
David GrunwaldRNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA, USA.ORCID http://orcid.org/0000-0001-9067-804X
Tingting GuUniversity of Oklahoma, Norman, OK, USA.ORCID http://orcid.org/0000-0002-0696-0008
Nadia HalidiAdvanced Light Microscopy Unit, Centre for Genomic Regulation, Barcelona, Spain.ORCID http://orcid.org/0000-0003-0613-2365
Mathias HammerRNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA, USA.ORCID http://orcid.org/0000-0002-5771-5694
Matthew HartleyEuropean Molecular Biology Laboratory (EMBL), European Bioinformatics Institute, Hinxton, UK.ORCID http://orcid.org/0000-0001-6178-2884
Marie HeldCentre for Cell Imaging, the University of Liverpool, Liverpool, UK.ORCID http://orcid.org/0000-0003-0118-5898
Florian JugFondazione Human Technopole, Milano, Italy.ORCID http://orcid.org/0000-0002-8499-5812
Varun KapoorDepartment of AI Research, Kapoor Labs, Paris, France.
Ayse Aslihan KoksoyMD Anderson Cancer Center, Houston, TX, USA.ORCID http://orcid.org/0000-0003-3092-5410
Judith LacosteMIA Cellavie Inc., Montreal, Quebec, Canada.ORCID http://orcid.org/0000-0002-8783-8599
Sylvia Le DévédecDivision of Drug Discovery and Safety, Cell Observatory, Leiden Academic Centre for Drug Research, Leiden University, Leiden, the Netherlands.ORCID http://orcid.org/0000-0002-0615-9616
Sylvie Le GuyaderKarolinska Institutet, Huddinge, Sweden.ORCID http://orcid.org/0000-0002-8812-8046
Penghuan LiuKey Laboratory for Modern Measurement Technology and Instruments of Zhejiang Province, College of Optical and Electronic Technology, China Jiliang University, Hangzhou, China.
Gabriel G MartinsAdvanced Imaging Facility, Instituto Gulbenkian de Ciência, Oeiras, Portugal.ORCID http://orcid.org/0000-0002-6506-9776
Aastha MathurEuro-BioImaging ERIC, Bio-Hub, Heidelberg, Germany.ORCID http://orcid.org/0000-0001-9734-9767
Kota MiuraBioimage Analysis and Research, Heidelberg, Germany.ORCID http://orcid.org/0000-0001-6926-191X
Paula Montero LlopisMicRoN Core, Harvard Medical School, Boston, MA, USA.ORCID http://orcid.org/0000-0002-5983-2296
Roland NitschkeLife Imaging Center, Signalling Research Centres CIBSS and BIOSS, University of Freiburg, Freiburg, Germany.
Alison NorthBio-Imaging Resource Center, the Rockefeller University, New York, NY, USA.
Adam C ParslowBaker Institute Microscopy Platform, Baker Heart and Diabetes Institute, Melbourne, Victoria, Australia.ORCID http://orcid.org/0000-0002-9868-6914
Alex Payne-DwyerSchool of Physics, Engineering and Technology, University of York, Heslington, UK.ORCID http://orcid.org/0000-0001-8802-352X
Laure PlantardFriedrich Miescher Institute for Biomedical Research, Basel, Switzerland.ORCID http://orcid.org/0000-0002-8386-3535
Rizwan AliKing Abdullah International Medical Research Center (KAIMRC), Medical Research Core Facility and Platforms (MRCFP), King Saud bin Abdulaziz University for Health Sciences (KSAU-HS), Ministry of National Guard Health Affairs, Riyadh, Saudi Arabia.
Britta Schroth-DiezLight Microscopy Facility, Max Planck Institute of Molecular Cell Biology and Genetics Dresden, Dresden, Germany.
Lucas Schützariadne.ai (Germany) GmbH, Heidelberg, Germany.
Ryan T ScottSpace Biosciences Division, NASA Ames Research Center, Moffett Field, CA, USA.ORCID http://orcid.org/0000-0003-0654-5661
Arne SeitzBioImaging and Optics Platform, Faculty of Life Sciences (SV), École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.ORCID http://orcid.org/0000-0002-5013-1557
Olaf SelchowMicroscopy and BioImaging Consulting, Image Processing and Large Data Handling, Gera, Germany.ORCID http://orcid.org/0009-0005-4110-9373
Ved P SharmaBio-Imaging Resource Center, the Rockefeller University, New York, NY, USA.ORCID http://orcid.org/0000-0001-9998-5070
Martin SpitalerMax Planck Institute of Biochemistry, Martinsried, Germany.ORCID http://orcid.org/0000-0002-8833-638X
Sathya SrinivasanImaging and Morphology Support Core, Oregon National Primate Research Center, OHSU West Campus, Beaverton, OR, USA.ORCID http://orcid.org/0000-0002-5386-8722
Caterina Strambio-De-CastilliaProgram in Molecular Medicine, University of Massachusetts Chan Medical School, Worcester, MA, USA.ORCID http://orcid.org/0000-0002-1069-1816
Douglas TaatjesDepartment of Pathology and Laboratory Medicine, Microscopy Imaging Center, Center for Biomedical Shared Resources, University of Vermont, Burlington, VT, USA.ORCID http://orcid.org/0000-0003-1903-983X
Christian TischerCentre for Bioimage Analysis, EMBL Heidelberg, Heidelberg, Germany. christian.tischer@embl.de.ORCID http://orcid.org/0000-0003-4105-1990
Helena Klara JamborNCT-UCC, Medizinische Fakultät TU Dresden, Dresden, Germany. helena.jambor@tu-dresden.de.ORCID http://orcid.org/0000-0003-3397-1842
Funding
4D Nucleome Network Data Coordination and Integration CenterU01CA200059 · NCI · HARVARD MEDICAL SCHOOL · PI PARK, PETER J · 2015 to 2024
$24.4MCenter for Open Bioimage AnalysisP41GM135019 · NIGMS · BROAD INSTITUTE, INC. · PI CARPENTER, ANNE E., CIMINI, BETH · 2020 to 2024
$6.9MMedical Research Council MR/W020793/1NCI NIH HHS U01 CA200059NIGMS NIH HHS P41 GM135019
Abstract
Images document scientific discoveries and are prevalent in modern biomedical research. Microscopy imaging in particular is currently undergoing rapid technological advancements. However, for scientists wishing to publish obtained images and image-analysis results, there are currently no unified guidelines for best practices. Consequently, microscopy images and image data in publications may be unclear or difficult to interpret. Here, we present community-developed checklists for preparing light microscopy images and describing image analyses for publications. These checklists offer authors, readers and publishers key recommendations for image formatting and annotation, color selection, data availability and reporting image-analysis workflows. The goal of our guidelines is to increase the clarity and reproducibility of image figures and thereby to heighten the quality and explanatory power of microscopy data.
Indexed as
ChecklistPublishingImage Processing, Computer-AssistedMicroscopyReproducibility of Results
Identifiers
What OpenQuestion holds
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Registered trials
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.