Evidence map›Paper›PMID 37708968›Full record

ArticleDevelopmental biology2023

Methods for dynamic and whole volume imaging of the zebrafish heart.

Isaac Bakis, Yuhan Sun, Laila Abd Elmagid, Xidi Feng, Mher Garibyan, Joycelyn K Yip, Fang Zhou Yu, Sayali Chowdhary, Gerardo Esteban Fernandez, Jingli Cao and 3 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
  3. Emerging Light-Sheet Fluorescence Microscopy for Cardiac Vascular Imaging in Zebrafish.Current treatment options in cardiovascular medicine · 2026
    Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Isaac BakisCardiovascular Research Institute, Weill Cornell Medicine, New York, NY, 10021, USA; Department of Cell and Developmental Biology, Weill Cornell Medicine, 1300 York Avenue, New York, NY, 10021, USA.
Yuhan SunSaban Research Institute and Heart Institute of Children's Hospital Los Angeles, Los Angeles, CA, 90027, USA.
Laila Abd ElmagidCardiovascular Research Institute, Weill Cornell Medicine, New York, NY, 10021, USA; Department of Cell and Developmental Biology, Weill Cornell Medicine, 1300 York Avenue, New York, NY, 10021, USA.
Xidi FengSaban Research Institute and Heart Institute of Children's Hospital Los Angeles, Los Angeles, CA, 90027, USA.
Mher GaribyanLaboratory for Living Systems Engineering, Alfred E. Mann Department of Biomedical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, CA, 90089, USA.
Joycelyn K YipLaboratory for Living Systems Engineering, Alfred E. Mann Department of Biomedical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, CA, 90089, USA.
Fang Zhou YuCardiovascular Research Institute, Weill Cornell Medicine, New York, NY, 10021, USA; Department of Emergency Medicine, Nuvance Health, Poughkeepsie, NY, 12601, USA.
Sayali ChowdharyCardiovascular Research Institute, Weill Cornell Medicine, New York, NY, 10021, USA; Department of Cell and Developmental Biology, Weill Cornell Medicine, 1300 York Avenue, New York, NY, 10021, USA.
Gerardo Esteban FernandezSaban Research Institute and Heart Institute of Children's Hospital Los Angeles, Los Angeles, CA, 90027, USA.
Jingli CaoCardiovascular Research Institute, Weill Cornell Medicine, New York, NY, 10021, USA; Department of Cell and Developmental Biology, Weill Cornell Medicine, 1300 York Avenue, New York, NY, 10021, USA.
Megan L McCainLaboratory for Living Systems Engineering, Alfred E. Mann Department of Biomedical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, CA, 90089, USA; Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, 90033, USA.
Ching-Ling LienSaban Research Institute and Heart Institute of Children's Hospital Los Angeles, Los Angeles, CA, 90027, USA; Department of Surgery, Keck School of Medicine, University of Southern California, Los Angeles, CA, 90033, USA. Electronic address: clien@chla.usc.edu.
Michael Rm HarrisonCardiovascular Research Institute, Weill Cornell Medicine, New York, NY, 10021, USA; Department of Cell and Developmental Biology, Weill Cornell Medicine, 1300 York Avenue, New York, NY, 10021, USA. Electronic address: mrh4003@med.cornell.edu.
Cornell University · USUniversity of Southern California · USChildren's Hospital of Los Angeles · US

Funding

Mechanisms controlling epicardial-dependent promotion of heart regeneration in zebrafish.R01HL155607 · NHLBI · WEILL MEDICAL COLL OF CORNELL UNIV · PI Jingli Cao · 2021 to 2026
$2.9M
Lymphatic Support of Neurogenesis and RegenerationR01NS126209 · NINDS · WEILL MEDICAL COLL OF CORNELL UNIV · PI Michael Harrison · 2023 to 2026
$1.8M
Cxc12 chemokine signaling regulates synchronous development of coronary vessels and myocardiumR01HL130172 · NHLBI · CHILDREN'S HOSPITAL OF LOS ANGELES · PI LIEN, CHING-LING E · 2017 to 2020
$1.7M
American Heart Association-American Stroke Association 941434NHLBI NIH HHS R01 HL130172NHLBI NIH HHS R01 HL155607NINDS NIH HHS R01 NS126209
6 · The paper itself

Abstract

Tissue development and regeneration are dynamic processes involving complex cell migration and cell-cell interactions. We have developed a protocol for complementary time-lapse and three-dimensional (3D) imaging of tissue for developmental and regeneration studies which we apply here to the zebrafish cardiac vasculature. 3D imaging of fixed specimens is used to first define the subject at high resolution then live imaging captures how it changes dynamically. Hearts from adult and juvenile zebrafish are extracted and cleaned in preparation for the different imaging modalities. For whole-mount 3D confocal imaging, single or multiple hearts with native fluorescence or immuno-labeling are prepared for stabilization or clearing, and then imaged. For live imaging, hearts are placed in a prefabricated fluidic device and set on a temperature-controlled microscope for culture and imaging over several days. This protocol allows complete visualization of morphogenic processes in a 3D context and provides the ability to follow cell behaviors to complement in vivo and fixed tissue studies. This culture and imaging protocol can be applied to different cell and tissue types. Here, we have used it to observe zebrafish coronary vasculature and the migration of coronary endothelial cells during heart regeneration.

Indexed as

Endothelial CellsZebrafishAnimalsHeartImaging, Three-DimensionalAntibody labelingClearingCoronary vesselsImagingLive-cell imagingRegenerationZebrafish

Identifiers

PMID37708968
PMCPMC10841891
OpenAlexW4386629846

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.