Evidence map›Paper›PMID 37706599›Full record

ArticleMolecular nutrition & food research2023

Alanine Scanning of the Unstructured Region of Ara h 2 and of a Related Mimotope Reveals Critical Amino Acids for IgE Binding.

Nicole Canon, Catherine H Schein, Werner Braun, Surendra S Negi, Xueni Chen, Michael D Kulis, Edwin H Kim, Vidya Pathy, Marina Pozzoli, Weimin Liu and 1 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular nutrition & food research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 1 country.

Nicole CanonDivision of Allergy and Immunology, Kelsey-Seybold Clinic, Houston, TX, 77010, USA.
Catherine H ScheinInstitute for Human Infections and Immunity (IHII), The University of Texas Medical Branch, Galveston, TX, 77555, USA.
Werner BraunSealy Center for Structural Biology and Molecular Biophysics, The University of Texas Medical Branch, Galveston, TX, 77555, USA.
Surendra S NegiSealy Center for Structural Biology and Molecular Biophysics, The University of Texas Medical Branch, Galveston, TX, 77555, USA.
Xueni ChenDivision of Allergy and Clinical Immunology, Department of Medicine, University of Colorado Denver, Aurora, CO, 80045, USA.
Michael D KulisDivision of Pediatric Allergy and Immunology, University of North Carolina School of Medicine, Chapel Hill, NC, 27599, USA.
Edwin H KimDivision of Pediatric Allergy and Immunology, University of North Carolina School of Medicine, Chapel Hill, NC, 27599, USA.
Vidya PathyDivision of Allergy and Clinical Immunology, Department of Medicine, University of Colorado Denver, Aurora, CO, 80045, USA.
Marina PozzoliDivision of Allergy and Clinical Immunology, Department of Medicine, University of Colorado Denver, Aurora, CO, 80045, USA.
Weimin LiuDivision of Allergy and Clinical Immunology, Department of Medicine, University of Colorado Denver, Aurora, CO, 80045, USA.
Stephen C DreskinDivision of Allergy and Clinical Immunology, Department of Medicine, University of Colorado Denver, Aurora, CO, 80045, USA.ORCID 0000-0003-3548-5530
University of Colorado Denver · USThe University of Texas Medical Branch at Galveston · USUniversity of North Carolina at Chapel Hill · USKelsey-Seybold Clinic · US

Funding

Exploiting and enhancing IgE-binding epitopes of the 2S albumins of peanuts and tree nutsR01AI165866 · NIAID · UNIVERSITY OF COLORADO DENVER · PI STEPHEN C DRESKIN · 2021 to 2026
$4.2M
Prediction and Classification of Allergenicity in a Protein FamilyR21AI109090 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI BRAUN, WERNER A · 2014 to 2015
$440k
Designing a mimotope-based vaccine for peanut allergyR21AI137881 · NIAID · UNIVERSITY OF COLORADO DENVER · PI CHEN, XUENI · 2019 to 2020
$428k
Designing peptides to block Ara h 2 and Ara h 6 from binding to IgER21AI135397 · NIAID · UNIVERSITY OF COLORADO DENVER · PI DRESKIN, STEPHEN C · 2019 to 2020
$427k
Characterizing and optimizing IgE and IgG4 microarray peptide assays for Ara h 2R03AI164349 · NIAID · UNIVERSITY OF COLORADO DENVER · PI DRESKIN, STEPHEN C · 2021 to 2022
$156k
NIAID NIH HHS R01 AI165866NIAID NIH HHS R03 AI164349NIAID NIH HHS R21 AI109090NIAID NIH HHS R21 AI135397NIAID NIH HHS R21 AI137881
6 · The paper itself

Abstract

scopeThe unstructured region of Ara h 2, referred to as epitope 3, contains a repeated motif, DYPSh (h = hydroxyproline) that is important for IgE binding. METHODS AND

resultsIgE binding assays to 20mer and shorter peptides of epitope 3, defines a 16mer core sequence containing one copy of the DPYSh motif, DEDSYERDPYShSQDP. This study performs alanine scanning of this and a related 12mer mimotope, LLDPYAhRAWTK. IgE binding, using a pool of 10 sera and with individual sera, is greatly reduced when alanine is substituted for aspartate at position 8 (D8; p < 0.01), tyrosine at position 10 (Y10; p < 0.01), and hydroxyproline at position 12 (h12; p < 0.001). IgE binding to alanine-substituted peptides of a mimotope containing the DPY_h motif confirm the critical importance of Y (p < 0.01) and h (p < 0.01), but not D. Molecular modeling of the core and mimotope suggests an h-dependent conformational basis for the recognition of these sequences by polyclonal IgE.

conclusionsIgE from pooled sera and individual sera differentially bound amino acids throughout the sequences of Epitope 3 and its mimotope, with Y10 and h12 being most important for all sera. These results are highly significant for designing hypoallergenic forms of Ara h 2.

Indexed as

Amino AcidsPeanut Hypersensitivity2S Albumins, PlantAlanineAllergensAmino Acid SequenceAntigens, PlantEpitopesHumansHydroxyprolineImmunoglobulin EPeptidesPlant Proteins2S Albumins, PlantAlanineAllergensAmino AcidsAntigens, PlantEpitopesHydroxyprolineImmunoglobulin EPeptidesPlant Proteins2S albuminsAra h 2epitopefood allergyIgEmimotopepeanutspeptides

Identifiers

PMID37706599
PMCPMC10840829
OpenAlexW4386726227

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.