Evidence map›Paper›PMID 37705997›Full record

ArticleFrontiers in chemistry2023

Deciphering the binding mechanism of an anti-cancer phytochemical plumbagin with calf thymus DNA using biophysical and

Abdul Rahaman, Farah Anjum, Aknita Kumari, Alaa Shafie, Mahafooj Alee, Omnia Badr, Shaheer Hasan Khan, Amal Adnan Ashour, Ali Hazazi, Sultan Arif and 1 more

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In one paragraph

Article in Frontiers in chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Abdul RahamanGuangdong Key Laboratory of Food Intelligent Manufacturing, Foshan University, Foshan, Guangdong, China.
Farah AnjumDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, Taif, Saudi Arabia.
Aknita KumariSchool of Food Science and Engineering, South China University of Technology, Guangzhou, China.
Alaa ShafieDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, Taif, Saudi Arabia.
Mahafooj AleeSchool of Food Science and Engineering, South China University of Technology, Guangzhou, China.
Omnia BadrDepartment of Genetics and Genetic Engineering, Faculty of Agriculture, Benha University, Qalyubia, Egypt.
Shaheer Hasan KhanEnzymology and Nanotechnology Laboratory, Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India.
Amal Adnan AshourDepartment of Oral and Maxillofacial Surgery and Diagnostic Sciences, Faculty of Dentistry, Taif University, Taif, Saudi Arabia.
Ali HazaziDepartment of Pathology and Laboratory Medicine, Security Forces Hospital Program, Riyadh, Saudi Arabia.
Sultan ArifDepartment of Plastic Surgery and Burn Unit, Security Forces Hospital, Riyadh, Saudi Arabia.
Xin-An ZengGuangdong Key Laboratory of Food Intelligent Manufacturing, Foshan University, Foshan, Guangdong, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Plumbagin (PLM), a plant derivative, is well known for a wide range of therapeutic effects in humans including anti-cancer, anti-inflammatory, anti-oxidant, and anti-microbial. Cytotoxic and genotoxic potential of this phytochemical has been studied which demands further insight. DNA being a major target for several drugs was taken to study against PLM to understand its effects on the cellular system. UV-Vis spectroscopy has indicated the binding of PLM to ctDNA and dye displacement assays have confirmed the formation of PLM-ctDNA complex. The insignificant changes in circular dichroism spectra suggested that PLM is not affecting the structural makeup of the ctDNA, hence the binding could be peripheral and not intercalating. Further, the relative viscosity and minimal change in melting temperature upon the complex formation supported this finding and confirmed the groove binding of PLM. Molecular docking analysis and simulation studies also show PLM as a minor groove binder to DNA and provide details on the interaction dynamics of PLM-DNA complex. Docking followed by a 100 ns simulation reveals the negative Gibbs free energy change (∆G = -6.6 kcal mol

Indexed as

biophysicalcancercircular dichroismgroove bindermolecular simulationPlumbagin

Identifiers

PMID37705997
PMCPMC10497110

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.