ArticleScientific reports2023
Rapid detection of tear lactoferrin for diagnosis of dry eyes by using fluorescence polarization-based aptasensor.
Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 23 citations in OpenAlex.
- Disease Biomarker Detection Using Fluorescence Polarization Assays: Principles and Clinical Applications.Diagnostics (Basel, Switzerland) · 2026Review
- Development of a Disposable Chemiresistive Biochip for Tear-Based Retinopathy of Prematurity Diagnosis: Early Screening and Risk Prediction.IEEE sensors journal · 2026Article
- Exploring TbThe journal of physical chemistry. B · 2025Article
- Carbon quantum dot-aptamer/MoSAnalytical and bioanalytical chemistry · 2025Article
- Translating Biomarker Discovery: From Bench to Bedside in Dry Eye Disease.International journal of molecular sciences · 2025Review
- Electroconductivity of Saliva as a Diagnostic Tool for Dry Eye Disease: A Case-Control Study.Translational vision science & technology · 2025Article
- Real-Time and Highly Sensitive Detection of Matrix Metalloproteinase 9 (MMP-9) in Tears Using Aptamer Functionalized Electrolyte-Gated IGZO Thin-Film Transistors.ACS applied materials & interfaces · 2025Article
- Dissolving Microneedles Containing Lactoferrin Nanosuspension for Enhancement of Antimicrobial and Anti-Inflammatory Effects in the Treatment of Dry Eye Disease.Pharmaceutics · 2025Article
- Determination of Lactoferrin Using High-Frequency Piezoelectric Quartz Aptamer Biosensor Based on Molecular Bond Rupture.Molecules (Basel, Switzerland) · 2024Article
- Development, Optimization, and Clinical Relevance of Lactoferrin Delivery Systems: A Focus on Ocular Delivery.Pharmaceutics · 2024Review
- Recommendations for nutritional supplements for dry eye disease: current advances.Frontiers in pharmacology · 2024Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Differentiating dry eye disease (DED) from allergic or viral conjunctivitis rapidly and accurately is important to ensure prompt diagnosis and treatment. Tear lactoferrin (LF), a multi-functional glycoprotein found in tears, decreases significantly in patients with DED, and has been considered as a DED diagnostic biomarker. Measuring tear LF level, however, takes time and requires the use of bulky instruments. Herein, a homogeneous carbon nanostructure-based aptasensor with high sensitivity and selectivity has been developed by applying fluorescence polarization (FP) technology. The FP of carbon dots (CDs) bioconjugated with LF aptamers (CDs-aptamer) is 21.2% higher than that of CDs, which can be further amplified (1.81 times) once interacting with graphene oxide nanosheets (GONS). In the presence of LF, GONS separates from CDs-aptamer because of the stronger binding affinity between CDs-aptamer to LF, resulting in the decrease of FP value. A linear relationship is observed between FP value and LF concentration in spiked tear samples from 0.66 to 3.32 mg/mL. The selectivity of the aptasensor has been investigated by measuring other proteins. The results indicate that the FP-based aptasensor is a cost-effective method with high sensitivity and selectivity in detection of tear LF.
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