ArticleNature communications2023
Facile hermetic TEM grid preparation for molecular imaging of hydrated biological samples at room temperature.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
7 citing papers in PubMed, 12 citations in OpenAlex.
- Room-temperature structure determination of vacuum-sensitive organic compounds by formvar encapsulation and serial electron diffraction.Journal of applied crystallography · 2025Article
- Graphene-Based Liquid Cell Designs for In Situ Liquid-Phase Transmission Electron Microscopy: Recent Developments and Perspectives.Small science · 2025Review
- Liquid Phase Biological Electron Microscopy: Many Published Results and Claimed Benefits Are Fantasy, Not Fact.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2025Article
- Imaging of Hydrated and Living Cells in Transmission Electron Microscope: Summary, Challenges, and Perspectives.ACS nano · 2025Review
- Unravelling complex mechanisms in materials processes with cryogenic electron microscopy.Chemical science · 2025Review
- Biosynthesis and characterization of iron oxide nanoparticles fabricated using cell-free supernatant of Pseudomonas fluorescens for antibacterial, antifungal, antioxidant, and photocatalytic applications.Scientific reports · 2025Article
- Liquid Phase Electron Microscopy of Bacterial Ultrastructure.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
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Authors and funding
10 authors at 5 institutions in 3 countries.
Funding
Abstract
Although structures of vitrified supramolecular complexes have been determined at near-atomic resolution, elucidating in situ molecular structure in living cells remains a challenge. Here, we report a straightforward liquid cell technique, originally developed for real-time visualization of dynamics at a liquid-gas interface using transmission electron microscopy, to image wet biological samples. Due to the scattering effects from the liquid phase, the micrographs display an amplitude contrast comparable to that observed in negatively stained samples. We succeed in resolving subunits within the protein complex GroEL imaged in a buffer solution at room temperature. Additionally, we capture various stages of virus cell entry, a process for which only sparse structural data exists due to their transient nature. To scrutinize the morphological details further, we used individual particle electron tomography for 3D reconstruction of each virus. These findings showcase this approach potential as an efficient, cost-effective complement to other microscopy technique in addressing biological questions at the molecular level.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.