ArticleProceedings of the National Academy of Sciences of the United States of America2023
Sequencing 4.3 million mutations in wheat promoters to understand and modify gene expression.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 22 citations in OpenAlex.
- The annotated blueprint: integrated functional genomic resources for a model tetraploid wheat Triticum turgidum cv Kronos.The New phytologist · 2026Article
- Spatial and single-cell expression analyses reveal complex expression domains in early wheat spike development.Genome biology · 2025Article
- The Annotated Blueprint: Integrated Functional Genomic Resources for a model Tetraploid WheatbioRxiv : the preprint server for biology · 2025Article
- Durum wheat mutants with enhanced disease resistance to stripe rust show differential responses to other fungal diseases.Molecular breeding : new strategies in plant improvement · 2025Article
- Genome-wide characterization and expression analysis of the CINNAMYL ALCOHOL DEHYDROGENASE gene family in Triticum aestivum.BMC genomics · 2024Article
- Epigenomic identification of vernalization cis-regulatory elements in winter wheat.Genome biology · 2024Article
- Stacked mutations in wheat homologues of rice SEMI-DWARF1 confer a novel semi-dwarf phenotype.BMC plant biology · 2024Article
- Sequencing 4.3 million mutations in wheat promoters to understand and modify gene expression.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
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Authors and funding
14 authors at 6 institutions in 3 countries.
Funding
Abstract
Wheat is an important contributor to global food security, and further improvements are required to feed a growing human population. Functional genetics and genomics tools can help us to understand the function of different genes and to engineer beneficial changes. In this study, we used a promoter capture assay to sequence 2-kb regions upstream of all high-confidence annotated genes from 1,513 mutagenized plants from the tetraploid wheat variety Kronos. We identified 4.3 million induced mutations with an accuracy of 99.8%, resulting in a mutation density of 41.9 mutations per kb. We also remapped Kronos exome capture reads to Chinese Spring RefSeq v1.1, identified 4.7 million mutations, and predicted their effects on annotated genes. Using these predictions, we identified 59% more nonsynonymous substitutions and 49% more truncation mutations than in the original study. To show the biological value of the promoter dataset, we selected two mutations within the promoter of the
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