Evidence map›Paper›PMID 37702715›Full record

ArticleJournal of proteome research2023

One-Pot Analytical Pipeline for Efficient and Sensitive Proteomic Analysis of Extracellular Vesicles.

Yi-Kai Liu, Xiaofeng Wu, Marco Hadisurya, Li Li, Hristos Kaimakliotis, Anton Iliuk, W Andy Tao

Open access · greenAbstract read
In one paragraph

Article in Journal of proteome research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Plasma-Derived Extracellular Vesicle Proteomics.Journal of proteome research · 2025
    Review
  6. Review
  7. Review
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Yi-Kai LiuDepartment of Biochemistry, Purdue University, West Lafayette, Indiana 47907, United States.
Xiaofeng WuDepartment of Chemistry, Purdue University, West Lafayette, Indiana 47907, United States.ORCID 0000-0002-8961-2163
Marco HadisuryaDepartment of Biochemistry, Purdue University, West Lafayette, Indiana 47907, United States.ORCID 0000-0002-4453-5854
Li LiTymora Analytical Operations, West Lafayette, Indiana 47906, United States.
Hristos KaimakliotisIndiana University School of Medicine, Indianapolis, Indiana 46202, United States.
Anton IliukDepartment of Biochemistry, Purdue University, West Lafayette, Indiana 47907, United States.
W Andy TaoDepartment of Biochemistry, Purdue University, West Lafayette, Indiana 47907, United States.ORCID 0000-0002-5535-5517
Purdue University West Lafayette · USIndiana University School of Medicine

Funding

Transgenic Mouse Core Facility Shared Resource (TMCF-SR)P30CA023168 · NCI · PURDUE UNIVERSITY WEST LAFAYETTE · PI John Tesmer · 1985 to 2026
$43.4M
Dissecting signaling pathways and seeking EV phosphoproteins as novel biomarkers for Alzheimer's DiseaseRF1AG064250 · NIA · PURDUE UNIVERSITY · PI TAO, W. ANDY · 2020 to 2021
$1.7M
Development of non-invasive biomarker discovery and diagnostics approach for bladder cancer based on urine proteome and phosphoproteomeR44CA239845 · NCI · TYMORA ANALYTICAL OPERATIONS, LLC · PI ILIUK, ANTON · 2019 to 2021
$1.5M
Dissecting signaling pathways and seeking EV phosphoproteins as novel biomarkers for Alzheimer's DiseaseR01AG064250 · NIA · PURDUE UNIVERSITY · PI TAO, W. ANDY · 2024 to 2024
$386k
NCI NIH HHS P30 CA023168NCI NIH HHS R44 CA239845NIA NIH HHS R01 AG064250NIA NIH HHS RF1 AG064250
6 · The paper itself

Abstract

Extracellular vesicle (EV) proteomics emerges as an effective tool for discovering potential biomarkers for disease diagnosis, monitoring, and therapeutics. However, the current workflow of mass spectrometry-based EV proteome analysis is not fully compatible in a clinical setting due to inefficient EV isolation methods and a tedious sample preparation process. To streamline and improve the efficiency of EV proteome analysis, here we introduce a one-pot analytical pipeline integrating a robust EV isolation approach, EV total recovery and purification (EVtrap), with

Indexed as

Extracellular VesiclesProteomeBiomarkersHumansMass SpectrometryProteomicsBiomarkersProteomebladder cancerEVtrapextracellular vesiclesproteomicsurine

Identifiers

PMID37702715
PMCPMC10897859
OpenAlexW4386704986

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.