Evidence map›Paper›PMID 37698609›Full record

ArticleApplied microbiology and biotechnology2023

Characterization of bifunctional alginate lyase Aly644 and antimicrobial activity of enzymatic hydrolysates.

Ao Zhang, Zhe Cao, Luying Zhao, Qian Zhang, Liping Fu, Jiang Li, Tao Liu

Abstract read
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In one paragraph

Article in Applied microbiology and biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
2.4field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 7 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Ao ZhangDepartment of Pharmaceutical Engineering, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China.
Zhe CaoKey Lab of Ecological Environment Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, 266061, China.
Luying ZhaoKey Lab of Ecological Environment Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, 266061, China.
Qian ZhangKey Lab of Ecological Environment Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, 266061, China.
Liping FuKey Lab of Ecological Environment Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, 266061, China.
Jiang LiKey Lab of Ecological Environment Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, 266061, China. lijiang@fio.org.cn.ORCID http://orcid.org/0000-0003-3608-0412
Tao LiuDepartment of Pharmaceutical Engineering, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China. taoyanghe@hotmail.com.
Ministry of Natural Resources · CNQingdao University of Science and Technology · CN

Funding

Impact and Response of Antarctic Seas to Climate Change IRFSOCC2023-2025
6 · The paper itself

Abstract

An alginate lyase gene aly644 encoding a member of polysaccharide lyase family 6 was obtained from a metagenome of Antarctic macroalgae-associated microbes. The gene was expressed heterologously in Escherichia coli, and the recombinant protein was purified using a Ni-NTA His Tag Kit. With sodium alginate as the substrate, recombinant Aly644 exhibited an optimum reaction temperature of 50°C and an optimum reaction pH of 7.0. The V

Indexed as

Anti-Infective AgentsPolysaccharide-LyasesAlginatesCloning, MolecularEnzyme StabilityEscherichia coliHydrogen-Ion ConcentrationHydrolysisKineticsMetagenomeOligosaccharidesPhytophthoraPolysaccharides, BacterialRecombinant ProteinsSeaweedSubstrate SpecificityAlginatesAnti-Infective AgentsOligosaccharidespoly(beta-D-mannuronate) lyasepolyguluronic acidPolysaccharide-LyasesPolysaccharides, BacterialRecombinant ProteinsAlginate lyaseAlginate oligosaccharideAntimicrobialEnzymatic propertiesPlant pathogen

Identifiers

PMID37698609
OpenAlexW4386624308

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.