Evidence map›Paper›PMID 37694610›Full record

ArticleDevelopment (Cambridge, England)2023

Chondroitin sulfate is required for follicle epithelial integrity and organ shape maintenance in Drosophila.

Collin Knudsen, Woo Seuk Koh, Tomomi Izumikawa, Eriko Nakato, Takuya Akiyama, Akiko Kinoshita-Toyoda, Greg Haugstad, Guichuan Yu, Hidenao Toyoda, Hiroshi Nakato

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Development (Cambridge, England), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.0field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Collin KnudsenDepartment of Genetics, Cell Biology, and Development, University of Minnesota at Twin Cities, Minneapolis, MN 55455, USA.ORCID 0000-0002-8250-3474
Woo Seuk KohDepartment of Genetics, Cell Biology, and Development, University of Minnesota at Twin Cities, Minneapolis, MN 55455, USA.
Tomomi IzumikawaFaculty of Pharmaceutical Sciences, Ritsumeikan University, Shiga 525-8577, Japan.
Eriko NakatoDepartment of Genetics, Cell Biology, and Development, University of Minnesota at Twin Cities, Minneapolis, MN 55455, USA.
Takuya AkiyamaStowers Institute for Medical Research, Kansas City, MO 64110, USA.
Akiko Kinoshita-ToyodaFaculty of Pharmaceutical Sciences, Ritsumeikan University, Shiga 525-8577, Japan.
Greg HaugstadCharacterization Facility, University of Minnesota at Twin Cities, Minneapolis, MN 55455, USA.
Guichuan YuCharacterization Facility, University of Minnesota at Twin Cities, Minneapolis, MN 55455, USA.
Hidenao ToyodaFaculty of Pharmaceutical Sciences, Ritsumeikan University, Shiga 525-8577, Japan.
Hiroshi NakatoDepartment of Genetics, Cell Biology, and Development, University of Minnesota at Twin Cities, Minneapolis, MN 55455, USA.ORCID 0000-0001-9812-4025
University of Minnesota · USRitsumeikan University · JPStowers Institute for Medical Research · US

Funding

Resource Component: Acquisition, maintenance and distribution of Drosophila stocksP40OD018537 · OD · TRUSTEES OF INDIANA UNIVERSITY · PI Annette L. Parks · 2014 to 2026
$13.5M
DROSOPHILA GENOMICS RESOURCE CENTERP40OD010949 · OD · TRUSTEES OF INDIANA UNIVERSITY · PI Andrew Zelhof · 2012 to 2026
$9.4M
Heparan sulfate proteoglycans in signaling and developmentR35GM131688 · NIGMS · UNIVERSITY OF MINNESOTA · PI Hiroshi Nakato · 2019 to 2026
$2.6M
An Interdisciplinary Training Program to Transform Graduate Education In Genetics and GenomicsT32GM140936 · NIGMS · UNIVERSITY OF MINNESOTA · PI ALBERT, FRANK WOLFGANG, GREENSTEIN, DAVID IRWIN · 2021 to 2025
$851k
NIGMS NIH HHS R35 GM131688NIGMS NIH HHS T32 GM140936NIH HHS P40 OD010949NIH HHS P40 OD018537
6 · The paper itself

Abstract

Heparan sulfate (HS) and chondroitin sulfate (CS) are evolutionarily conserved glycosaminoglycans that are found in most animal species, including the genetically tractable model organism Drosophila. In contrast to extensive in vivo studies elucidating co-receptor functions of Drosophila HS proteoglycans (PGs), only a limited number of studies have been conducted for those of CSPGs. To investigate the global function of CS in development, we generated mutants for Chondroitin sulfate synthase (Chsy), which encodes the Drosophila homolog of mammalian chondroitin synthase 1, a crucial CS biosynthetic enzyme. Our characterizations of the Chsy mutants indicated that a fraction survive to adult stage, which allowed us to analyze the morphology of the adult organs. In the ovary, Chsy mutants exhibited altered stiffness of the basement membrane and muscle dysfunction, leading to a gradual degradation of the gross organ structure as mutant animals aged. Our observations show that normal CS function is required for the maintenance of the structural integrity of the ECM and gross organ architecture.

Indexed as

Chondroitin SulfatesDrosophilaAnimalsFemaleGlycosaminoglycansMammalsOvarian FollicleOvaryChondroitin SulfatesGlycosaminoglycansBasement membraneChondroitin sulfateChsyDrosophilaMuscle sheathOvary

Identifiers

PMID37694610
PMCPMC10508698
OpenAlexW4386593476

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.