Evidence map›Paper›PMID 37693615›Full record

ArticlebioRxiv : the preprint server for biology2023

ROM1 is redundant to PRPH2 as a molecular building block of photoreceptor disc rims.

Tylor R Lewis, Mustafa S Makia, Carson M Castillo, Ying Hao, Muayyad R Al-Ubaidi, Nikolai P Skiba, Shannon M Conley, Vadim Y Arshavsky, Muna I Naash

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Tylor R LewisDepartment of Ophthalmology, Duke University Medical Center, Durham, NC, USA, 27710.
Mustafa S MakiaDepartment of Biomedical Engineering, University of Houston, Houston, TX, USA, 77204.
Carson M CastilloDepartment of Ophthalmology, Duke University Medical Center, Durham, NC, USA, 27710.
Ying HaoDepartment of Ophthalmology, Duke University Medical Center, Durham, NC, USA, 27710.
Muayyad R Al-UbaidiDepartment of Biomedical Engineering, University of Houston, Houston, TX, USA, 77204.
Nikolai P SkibaDepartment of Ophthalmology, Duke University Medical Center, Durham, NC, USA, 27710.
Shannon M ConleyDepartment of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA, 73104.
Vadim Y ArshavskyDepartment of Ophthalmology, Duke University Medical Center, Durham, NC, USA, 27710.
Muna I NaashDepartment of Biomedical Engineering, University of Houston, Houston, TX, USA, 77204.
Duke Medical Center · USUniversity of Houston · USUniversity of Oklahoma Health Sciences Center · US

Funding

VISION RESEARCHP30EY005722 · NEI · DUKE UNIVERSITY · PI Vadim Y Arshavsky · 1985 to 2026
$19.3M
Supplement for: Mechanism of Photoreceptor Cell Degeneration in Animal Models of Retinal DiseasesR01EY010609 · NEI · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI MUAYYAD R AL-UBAIDI, Muna I. Naash · 1995 to 2026
$11.0M
Mechanisms of photoreceptor disc maturationR01EY030451 · NEI · DUKE UNIVERSITY · PI ARSHAVSKY, VADIM Y · 2020 to 2024
$2.4M
The role of IGF-1 signaling in vascular smooth muscle cells in age-related vascular cognitive impairment and dementiaR01AG070915 · NIA · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI CONLEY, SHANNON M · 2021 to 2025
$1.8M
Non-viral gene delivery platforms for the treatment of Usher Syndrome Type 2A.R01EY034671 · NEI · UNIVERSITY OF HOUSTON · PI Muna I. Naash · 2023 to 2026
$1.7M
Mechanism of photoreceptor cell degeneration in animal models of retinal diseasesR56EY010609 · NEI · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI NAASH, MUNA I. · 2008 to 2008
$479k
The role of ADAM9 in retinal health and diseaseK99EY033763 · NEI · DUKE UNIVERSITY · PI LEWIS, TYLOR ROBERT · 2022 to 2023
$252k
NEI NIH HHS K99 EY033763NEI NIH HHS P30 EY005722NEI NIH HHS R01 EY010609NEI NIH HHS R01 EY030451NEI NIH HHS R01 EY034671NEI NIH HHS R56 EY010609NIA NIH HHS R01 AG070915
6 · The paper itself

Abstract

Visual signal transduction takes place within a stack of flattened membranous "discs" enclosed within the light-sensitive photoreceptor outer segment. The highly curved rims of these discs, formed in the process of disc enclosure, are fortified by large hetero-oligomeric complexes of two homologous tetraspanin proteins, PRPH2 (a.k.a. peripherin-2 or rds) and ROM1. While mutations in PRPH2 affect the formation of disc rims, the role of ROM1 remains poorly understood. In this study, we found that the knockout of ROM1 causes a compensatory increase in the disc content of PRPH2. Despite this increase, discs of ROM1 knockout mice displayed a delay in disc enclosure associated with a large diameter and lack of incisures in mature discs. Strikingly, further increasing the level of PRPH2 rescued these morphological defects. We next showed that disc rims are still formed in a knockin mouse in which the tetraspanin body of PRPH2 was replaced with that of ROM1. Together, these results demonstrate that, despite its contribution to the formation of disc rims, ROM1 can be replaced by an excess of PRPH2 for timely enclosure of newly forming discs and establishing normal outer segment structure.

Indexed as

ciliaouter segmentphotoreceptorretinatetraspaninvision

Identifiers

PMID37693615
PMCPMC10491102
OpenAlexW4382893363

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.