Evidence map›Paper›PMID 37692384›Full record

ReviewFrontiers in microbiology2023

Opportunities in optical and electrical single-cell technologies to study microbial ecosystems.

Fabian Mermans, Valérie Mattelin, Ruben Van den Eeckhoudt, Cristina García-Timermans, Josefien Van Landuyt, Yuting Guo, Irene Taurino, Filip Tavernier, Michael Kraft, Hira Khan and 1 more

Abstract readReview
In one paragraph

Review in Frontiers in microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Raman cell sorting for single-cell research.Frontiers in bioengineering and biotechnology · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Fabian MermansCenter for Microbial Ecology and Technology (CMET), Department of Biotechnology, Ghent University, Ghent, Belgium.
Valérie MattelinCenter for Microbial Ecology and Technology (CMET), Department of Biotechnology, Ghent University, Ghent, Belgium.
Ruben Van den EeckhoudtMicro- and Nanosystems (MNS), Department of Electrical Engineering (ESAT), KU Leuven, Leuven, Belgium.
Cristina García-TimermansCenter for Microbial Ecology and Technology (CMET), Department of Biotechnology, Ghent University, Ghent, Belgium.
Josefien Van LanduytCenter for Microbial Ecology and Technology (CMET), Department of Biotechnology, Ghent University, Ghent, Belgium.
Yuting GuoCenter for Microbial Ecology and Technology (CMET), Department of Biotechnology, Ghent University, Ghent, Belgium.
Irene TaurinoMicro- and Nanosystems (MNS), Department of Electrical Engineering (ESAT), KU Leuven, Leuven, Belgium.
Filip TavernierMICAS, Department of Electrical Engineering (ESAT), KU Leuven, Leuven, Belgium.
Michael KraftMicro- and Nanosystems (MNS), Department of Electrical Engineering (ESAT), KU Leuven, Leuven, Belgium.
Hira KhanCenter for Microbial Ecology and Technology (CMET), Department of Biotechnology, Ghent University, Ghent, Belgium.
Nico BoonCenter for Microbial Ecology and Technology (CMET), Department of Biotechnology, Ghent University, Ghent, Belgium.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

New techniques are revolutionizing single-cell research, allowing us to study microbes at unprecedented scales and in unparalleled depth. This review highlights the state-of-the-art technologies in single-cell analysis in microbial ecology applications, with particular attention to both optical tools, i.e., specialized use of flow cytometry and Raman spectroscopy and emerging electrical techniques. The objectives of this review include showcasing the diversity of single-cell optical approaches for studying microbiological phenomena, highlighting successful applications in understanding microbial systems, discussing emerging techniques, and encouraging the combination of established and novel approaches to address research questions. The review aims to answer key questions such as how single-cell approaches have advanced our understanding of individual and interacting cells, how they have been used to study uncultured microbes, which new analysis tools will become widespread, and how they contribute to our knowledge of ecological interactions.

Indexed as

CMOS-MEAelectrical techniquesflow cytometryimpedance flow cytometrymicrobial ecologyoptical techniquesRaman spectroscopysingle-cell

Identifiers

PMID37692384
PMCPMC10486927

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.