Evidence map›Paper›PMID 37692237›Full record

ArticleACS omega2023

Miniaturized Microscale Solid-Phase Extraction-Based Module for Highly Efficient DNA Extraction and Purification from Grapevine Samples.

Carla Teixeira, Andrey Ipatov, Joana Carvalho, Agnes Purwidyantri, Natacha Fontes, Marta Prado

Open access · goldAbstract read
In one paragraph

Article in ACS omega, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.6field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 5 institutions in 3 countries.

Carla TeixeiraInternational Iberian Nanotechnology Laboratory (INL), Avenida Mestre José Veiga, 4715-310 Braga, Portugal.ORCID https://orcid.org/0000-0001-7241-493X
Andrey IpatovInternational Iberian Nanotechnology Laboratory (INL), Avenida Mestre José Veiga, 4715-310 Braga, Portugal.
Joana CarvalhoInternational Iberian Nanotechnology Laboratory (INL), Avenida Mestre José Veiga, 4715-310 Braga, Portugal.
Agnes PurwidyantriInternational Iberian Nanotechnology Laboratory (INL), Avenida Mestre José Veiga, 4715-310 Braga, Portugal.
Natacha FontesSogrape, Rua 5 de outubro, 4527, 4430-809 Avintes, Portugal.
Marta PradoInternational Iberian Nanotechnology Laboratory (INL), Avenida Mestre José Veiga, 4715-310 Braga, Portugal.ORCID https://orcid.org/0000-0002-0529-5771
International Iberian Nanotechnology Laboratory · PTQueen's University Belfast · GBSogrape Vinhos (Portugal) · PTUniversidade de Santiago de Compostela · ESUniversidade do Porto · PT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Several developments over the last few years are being directed toward improving DNA-based analysis to simplify, miniaturize, and reduce the time and cost of analysis, with the objective to allow its use in decentralized settings. One of the most interesting fields is DNA extraction and purification, a key step for ensuring good analytical performance. In this sense, microscale solid phase extraction (μSPE) offers paramount advantages for an improved DNA yield. In this work, we have developed a miniaturized module for DNA purification based on μSPE using a borosilicate glass microfiber filter as the solid phase. We also established a protocol for highly efficient DNA purification from vegetable samples, including leaves and grapes from four different varieties from the PDO Douro and two varieties from the Minho wine regions. The protocol demonstrated excellent performance when compared with a commercial kit with a DNA recovery yield of around 50%.

Identifiers

PMID37692237
PMCPMC10483517
OpenAlexW4386142182

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.