ArticleBMC plant biology2023
The role of WRKY transcription factors, FaWRKY29 and FaWRKY64, for regulating Botrytis fruit rot resistance in strawberry (Fragaria × ananassa Duch.).
Article in BMC plant biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- WRKY transcription factors in horticultural crops: regulating quality traits and fruit stress responses.Planta · 2026Review
- Genome-Wide Identification of NLP Family Genes in Cultivated Strawberry (International journal of molecular sciences · 2026Article
- Rapid screening of genome edited strawberry (Fragaria ×ananassa) regenerants using high-resolution melting analysis followed by Amplicon sequencing.BMC research notes · 2026Article
- Genome-Wide Identification of WRKY Group II Genes and the Role ofPlants (Basel, Switzerland) · 2026Article
- Genome-wide association study of exotic Fragaria germplasm accessions for resistance to Phytophthora crown rot in strawberry.BMC plant biology · 2026Article
- The shallow domestication bottleneck of strawberry: recovering secondary metabolite-based defenses from wildFrontiers in plant science · 2026Review
- Whitening fruit by CRISPR/Cas9-mediated homoeolog-specific gene editing ofHorticulture research · 2026Article
- FaMYB63 and FvWYRKY75 Activate FvPR10.14 Boosting Strawberry Immunity Against Powdery Mildew.Molecular plant pathology · 2025Article
- Genomic resources and genetic improvement of vital tropical and subtropical fruit crops: current status and prospects.AoB PLANTS · 2025Review
- Winning by Losing: Exploiting Modified Plant Susceptibility Genes to Counteract Necrotrophic Fungal Pathogens.Plant biotechnology journal · 2025Review
- A loss-of-function of ZmWRKY125 induced by CRISPR/Cas9 improves resistance against Fusarium verticillioides in maize kernels.Plant cell reports · 2025Article
- Combined transcriptome and metabolome analysis ofFrontiers in plant science · 2025Article
- FaNPR3 Members of the NPR1-like Gene Family Negatively Modulate Strawberry Fruit Resistance againstPlants (Basel, Switzerland) · 2024Article
- Challenges and Opportunities Arising from Host-International journal of molecular sciences · 2024Review
- Tomato SlWRKY3 Negatively RegulatesPlants (Basel, Switzerland) · 2024Article
- Advances in genomics and genome editing for improving strawberry (Frontiers in genetics · 2024Review
- Article
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
backgroundThe cultivated strawberry (Fragaria × ananassa Duch.) is one of the most economically important horticultural crops worldwide. Botrytis fruit rot (BFR) caused by the necrotrophic fungal pathogen Botrytis cinerea is the most devasting disease of cultivated strawberries. Most commercially grown strawberry varieties are susceptible to BFR, and controlling BFR relies on repeated applications of various fungicides. Despite extensive efforts, breeding for BFR resistance has been unsuccessful, primarily due to lack of information regarding the mechanisms of disease resistance and genetic resources available in strawberry.
resultsUsing a reverse genetics approach, we identified candidate genes associated with BFR resistance and screened Arabidopsis mutants using strawberry isolates of B. cinerea. Among the five Arabidopsis T-DNA knockout lines tested, the mutant line with AtWRKY53 showed the greatest reduction in disease symptoms of BFR against the pathogen. Two genes, FaWRKY29 and FaWRKY64, were identified as orthologs in the latest octoploid strawberry genome, 'Florida Brilliance'. We performed RNAi-mediated transient assay and found that the disease frequencies were significantly decreased in both FaWRKY29- and FaWRKY64-RNAi fruits of the strawberry cultivar, 'Florida Brilliance'. Furthermore, our transcriptomic data analysis revealed significant regulation of genes associated with ABA and JA signaling, plant cell wall composition, and ROS in FaWRKY29 or FaWRKY64 knockdown strawberry fruits in response to the pathogen.
conclusionOur study uncovered the foundational role of WRKY transcription factor genes, FaWRKY29 and FaWRKY64, in conferring resistance against B. cinerea. The discovery of susceptibility genes involved in BFR presents significant potential for developing resistance breeding strategies in cultivated strawberries, potentially leveraging CRISPR-based gene editing techniques.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.