Evidence map›Paper›PMID 37689689›Full record

ArticleJournal of neuroinflammation2023

Assessing the role of T cells in response to retinal injury to uncover new therapeutic targets for the treatment of retinal degeneration.

Federica M Conedera, Judith M Runnels, Jens V Stein, Clemens Alt, Volker Enzmann, Charles P Lin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neuroinflammation, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
2.3field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 19 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 5 institutions in 2 countries.

Federica M ConederaDepartment of Oncology, Microbiology and Immunology, University of Fribourg, Fribourg, Switzerland.
Judith M RunnelsCenter for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.
Jens V SteinDepartment of Oncology, Microbiology and Immunology, University of Fribourg, Fribourg, Switzerland.
Clemens Alt *Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.
Volker Enzmann *Department of Ophthalmology, Bern University Hospital, Bern, Switzerland. Volker.Enzmann@insel.ch.
Charles P Lin *Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.
Center for Systems Biology · USHarvard University · USUniversity Hospital of Bern · CHUniversity of Bern · CHUniversity of Fribourg · CH

Funding

Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung P2BEP3_191779Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung P500PB_203039
6 · The paper itself

Abstract

backgroundRetinal degeneration is a disease affecting the eye, which is an immune-privileged site because of its anatomical and physiological properties. Alterations in retinal homeostasis-because of injury, disease, or aging-initiate inflammatory cascades, where peripheral leukocytes (PL) infiltrate the parenchyma, leading to retinal degeneration. So far, research on PL's role in retinal degeneration was limited to observing a few cell types at specific times or sectioning the tissue. This restricted our understanding of immune cell interactions and response duration.

methodsIn vivo microscopy in preclinical mouse models can overcome these limitations enabling the spatio-temporal characterization of PL dynamics. Through in vivo imaging, we assessed structural and fluorescence changes in response to a focal injury at a defined location over time. We also utilized minimally invasive techniques, pharmacological interventions, and knockout (KO) mice to determine the role of PL in local inflammation. Furthermore, we investigated PL abundance and localization during retinal degeneration in human eyes by histological analysis to assess to which extent our preclinical study translates to human retinal degeneration.

resultsWe demonstrate that PL, especially T cells, play a detrimental role during retinal injury response. In mice, we observed the recruitment of helper and cytotoxic T cells in the parenchyma post-injury, and T cells also resided in the macula and peripheral retina in pathological conditions in humans. Additionally, we found that the pharmacological PL reduction and genetic depletion of T-cells reduced injured areas in murine retinas and rescued the blood-retina barrier (BRB) integrity. Both conditions promoted morphological changes of Cx3cr1

conclusionsOur data provide novel insights into the adaptive immune response to retinal injury in mice and human retinal degeneration. Such information is fundamental to understanding retinal disorders and developing therapeutics to modulate immune responses to retinal degeneration safely.

Indexed as

Retinal DegenerationAgingAnimalsCD8-Positive T-LymphocytesHumansLeukocytesMiceRetinaAdaptive immune cellsCD8+ T cellsHumanIn vivo imagingMicrogliaMouseRetinal laser-injury

Identifiers

PMID37689689
PMCPMC10492418
OpenAlexW4386572663

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.