Evidence map›Paper›PMID 37688732›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Identification and Localization of Cell Types in the Mouse Olfactory Bulb Using Slide-SeqV2.

Ai Fang, Kaitlyn Petentler, Andrew Price, Seth Malloy, Michael Peterson, Lucinda Maddera, Jonathon Russell, McKenzie Treese, Hua Li, Yongfu Wang and 3 more

Erratum issuedAbstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors.

Ai FangStowers Institute for Medical Research, Kansas City, MO, USA.
Kaitlyn PetentlerStowers Institute for Medical Research, Kansas City, MO, USA.
Andrew PriceStowers Institute for Medical Research, Kansas City, MO, USA.
Seth MalloyStowers Institute for Medical Research, Kansas City, MO, USA.
Michael PetersonStowers Institute for Medical Research, Kansas City, MO, USA.
Lucinda MadderaStowers Institute for Medical Research, Kansas City, MO, USA.
Jonathon RussellStowers Institute for Medical Research, Kansas City, MO, USA.
McKenzie TreeseStowers Institute for Medical Research, Kansas City, MO, USA.
Hua LiStowers Institute for Medical Research, Kansas City, MO, USA.
Yongfu WangStowers Institute for Medical Research, Kansas City, MO, USA.
Sean McKinneyStowers Institute for Medical Research, Kansas City, MO, USA.
Anoja PereraStowers Institute for Medical Research, Kansas City, MO, USA.
C Ron YuStowers Institute for Medical Research, Kansas City, MO, USA. cry@stowers.org.

Funding

Circuit architecture and dynamics representation in odor perceptionR01DC014701 · NIDCD · STOWERS INSTITUTE FOR MEDICAL RESEARCH · PI Thomas A Cleland, Yingxi Lin · 2015 to 2026
$6.4M
Mechanisms of Developmental Plasticity in the Mammalian Olfactory SystemR01DC016696 · NIDCD · STOWERS INSTITUTE FOR MEDICAL RESEARCH · PI Congrong Ron Yu · 2018 to 2026
$3.9M
NIDCD NIH HHS R01 DC014701NIDCD NIH HHS R01 DC016696
6 · The paper itself

Abstract

Spatial transcriptomics maps RNA molecules to the location in a tissue where they are expressed. Here we document the use of Slide-SeqV2 to visualize gene expression in the mouse olfactory bulb (OB). This approach relies on spatially identified beads to locate and quantify individual transcripts. The expression profiles associated with the beads are used to identify and localize individual cell types in an unbiased manner. We demonstrate the various cell types and subtypes with distinct spatial locations in the olfactory bulb that are identified using Slide-SeqV2.

Indexed as

Gene Expression ProfilingOlfactory BulbAnimalsMiceGABAergic interneuronsMitral and tufted cellsMouseNeural developmentOlfactory bulbSlide-SeqV2Spatial transcriptome

Identifiers

PMID37688732
PMCPMC11061798

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.