Evidence map›Paper›PMID 37688720›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Visualizing and Manipulating Olfactory Cilia Through Viral Delivery Coupled with En Face Imaging of Intact OE.

Julien C Habif, Chao Xie, Jeffrey R Martens

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 55% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Julien C HabifDepartment of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville, FL, USA.
Chao XieDepartment of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville, FL, USA.
Jeffrey R MartensDepartment of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville, FL, USA. martensj@ufl.edu.
Taste and Smell Clinic · USUniversity of Florida · US

Funding

Maintenance and Disassembly of Olfactory CiliaR01DC019345 · NIDCD · UNIVERSITY OF VIRGINIA · PI MARTENS, JEFFREY · 2021 to 2025
$2.1M
Training Program in Chemosensory ScienceT32DC015994 · NIDCD · UNIVERSITY OF FLORIDA · PI Daniel W Wesson · 2018 to 2026
$1.4M
A role of ARL13B in the control of OSN innervation of the olfactory bulbF31DC020638 · NIDCD · UNIVERSITY OF FLORIDA · PI HABIF, JULIEN · 2022 to 2022
$41k
NIDCD NIH HHS F31 DC020638NIDCD NIH HHS R01 DC019345NIDCD NIH HHS T32 DC015994
6 · The paper itself

Abstract

Olfactory cilia are the obligate transducers of the odorant signal, and thus their study has been a focus of investigation in the olfactory field. Various methodologies have been established to visualize the cilia of olfactory sensory neurons; however, these approaches are limited to static imaging and often lack the ability to resolve individual cilia projecting from solitary neurons in the postnatal mouse. Here we detail a procedure of the visualization of olfactory cilia by ectopic expression of fluorescently tagged proteins. The procedure can be used for the observation and manipulation of the olfactory cilia and ciliary proteins in both static and dynamic conditions.

Indexed as

CiliaOlfactory Receptor NeuronsAnimalsDiffusion Magnetic Resonance ImagingMiceOdorantsSmellCiliaEn face imagingFRAPIntranasal deliveryOlfactory epitheliumTIRF

Identifiers

PMID37688720
OpenAlexW4386569129

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.